The objective of this study was to use microplate immunocapture (IC) to reduce the enrichment time required for detection of Salmonella in pet food with the 3 M Molecular Detection System (MDS) or selective plating on XLD. Dog food and pig ear treats were inoculated with Salmonella Infantis at concentrations of 100-104 CFU/25 g, followed by a 3-h enrichment, then microplate IC and 3 M MDS or microplate IC and selective plating on XLD. Another set of samples underwent a traditional 24-h enrichment followed by 3 M MDS or selective plating. Based on the results of three independent trials, microplate IC followed by selective plating enabled detection of Salmonella in 100% of dog food and treat samples tested, including at levels as low as 100 CFU/25 g. Microplate IC coupled with 3 M MDS enabled detection of Salmonella in dog food and treat samples down to levels of 100 CFU/25 g, with an overall detection rate of 92%. These results indicate high potential for microplate IC to be used in place of the traditional 24-h enrichment step, enabling detection of Salmonella in complex matrices when coupled with 3 M MDS or selective plating.

中文翻译：

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微孔板免疫捕获技术结合3M分子检测系统和选择性电镀技术，可快速检测干狗粮和零食中的婴儿沙门氏菌。

这项研究的目的是使用微孔板免疫捕获（IC）来减少通过3 M分子检测系统（MDS）或XLD上的选择性电镀检测宠物食品中沙门氏菌所需的富集时间。用沙门氏菌沙门氏菌（Salmonella Infantis）接种浓度为100-104 CFU / 25 g的狗粮和猪耳零食，然后浓缩3小时，然后加入酶标板IC和3 M MDS或酶标板IC，并在XLD上进行选择性铺板。另一组样品经过传统的24小时浓缩，然后进行3 M MDS或选择性平板接种。根据三项独立试验的结果，微孔板IC和选择性孔板能够在100％的狗粮中检测沙门氏菌，并处理低至100 CFU / 25 g的样品。微孔板IC结合3 M MDS可以检测狗食中的沙门氏菌，并处理低至100 CFU / 25 g的样品，总检测率为92％。这些结果表明，微孔板IC可以代替传统的24小时富集步骤，具有很高的潜力，当与3 M MDS或选择性电镀结合使用时，能够检测复杂基质中的沙门氏菌。