Heat shock proteins (Hsp) are among highly conserved proteins across all domains of life. Though originally discovered as a cellular response to stress, these proteins are also involved in a wide range of cellular functions such as protein refolding, protein trafficking and cellular signalling. A large number of potential Hsp modulators are under clinical trials against various human diseases. As the number of modulators targeting Hsps is growing, there is a need to develop a comprehensive knowledge repository of these findings which is largely scattered. We have thus developed a web-accessible database, HSPMdb, which is a first of its kind manually curated repository of experimentally validated Hsp modulators (activators and inhibitors). The data was collected from 176 research articles and current version of HSPMdb holds 10 223 entries of compounds that are known to modulate activities of five major Hsps (Hsp100, Hsp90, Hsp70, Hsp60 and Hsp40) originated from 15 different organisms (i.e. human, yeast, bacteria, virus, mouse, rat, bovine, porcine, canine, chicken, Trypanosoma brucei and Plasmodium falciparum). HSPMdb provides comprehensive information on biological activities as well as the chemical properties of Hsp modulators. The biological activities of modulators are presented as enzymatic activity and cellular activity. Under the enzymatic activity field, parameters such as IC50, EC50, DC50, Ki and KD have been provided. In the cellular activity field, complete information on cellular activities (percentage cell growth inhibition, EC50 and GI50), type of cell viability assays and cell line used has been provided. One of the important features of HSPMdb is that it allows users to screen whether or not their compound of interest has any similarity with the previously known Hsp modulators. We anticipate that HSPMdb would become a valuable resource for the broader scientific community working in the area of chaperone biology and protein misfolding diseases. HSPMdb is freely accessible at http://bioinfo.imtech.res.in/bvs/hspmdb/index.php.

中文翻译：

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HSPMdb：热激蛋白调节剂的计算库。

热休克蛋白（Hsp）是生命各个领域中高度保守的蛋白之一。尽管最初发现这些蛋白质是对压力的细胞反应，但这些蛋白质还涉及多种细胞功能，例如蛋白质复性，蛋白质运输和细胞信号传导。大量潜在的Hsp调节剂正在针对各种人类疾病的临床试验中。随着靶向Hsps的调节剂的数量不断增长，有必要针对这些发现建立一个广泛分散的综合知识库。因此，我们开发了一个可通过Web访问的数据库HSPMdb，这是同类中第一个通过实验验证的Hsp调节剂（活化剂和抑制剂）手动整理的存储库。数据收集自176篇研究文章，HSPMdb的当前版本包含10 223种化合物，这些化合物已知可调节源自15种不同生物（即人类，酵母菌）的五种主要Hsps（Hsp100，Hsp90，Hsp70，Hsp60和Hsp40）的活性，细菌，病毒，小鼠，大鼠，牛，猪，犬，鸡，布鲁氏锥虫和恶性疟原虫）。HSPMdb提供有关生物活性以及Hsp调节剂的化学性质的全面信息。调节剂的生物活性表示为酶活性和细胞活性。在酶活性字段下，提供了诸如IC50，EC50，DC50，Ki和KD之类的参数。在细胞活性字段中，填写有关细胞活性的信息（细胞生长抑制百分比，EC50和GI50），已经提供了细胞活力测定法和所用细胞系的类型。HSPMdb的重要功能之一是允许用户筛选其感兴趣的化合物是否与以前已知的Hsp调节剂相似。我们期望HSPMdb将成为在分子伴侣生物学和蛋白质错误折叠疾病领域工作的更广泛的科学界的宝贵资源。HSPMdb可从http://bioinfo.imtech.res.in/bvs/hspmdb/index.php免费访问。我们期望HSPMdb将成为在分子伴侣生物学和蛋白质错误折叠疾病领域工作的更广泛的科学界的宝贵资源。HSPMdb可从http://bioinfo.imtech.res.in/bvs/hspmdb/index.php免费访问。我们期望HSPMdb将成为在分子伴侣生物学和蛋白质错误折叠疾病领域工作的更广泛的科学界的宝贵资源。HSPMdb可从http://bioinfo.imtech.res.in/bvs/hspmdb/index.php免费访问。