The Nuclear factor I proteins comprise a family of transcription factors that are expressed in many developing and mature cell populations, including within the central nervous system. Within the embryonic mouse spinal cord, NFIA and NFIB are expressed by neural progenitor cells lining the central canal, where they act to promote astrocytic and oligodendrocytic lineage specification. Cells lining the mature spinal cord central canal retain characteristics of neural progenitor cells, but the expression of NFIA and NFIB within the mature spinal cord at a cell-type-specific level remains undefined. Here, we investigated where these two transcription factors are expressed within the adult mouse spinal cord. We reveal that both factors are expressed in similar cohorts of mature cells, including ependymal cells, interneurons and motor neurons. We also show robust and widespread expression of NFIA and NFIB within nestin-expressing cells following injury to the spinal cord. Collectively, these data provide a basis to further define what functional role(s) NFIA and NFIB play within the adult spinal cord.

核因子I蛋白包含一个转录因子家族，在许多发育中和成熟的细胞群体（包括中枢神经系统内）中表达。在胚胎小鼠脊髓中，NFIA和NFIB由位于中央管内的神经祖细胞表达，它们在细胞中起到促进星形胶质细胞和少突胶质细胞谱系规范的作用。位于成熟脊髓中央管内的细胞保留了神经祖细胞的特征，但是在成熟脊髓中以细胞类型特异性水平表达NFIA和NFIB仍然不确定。在这里，我们调查了成年小鼠脊髓中这两个转录因子的表达情况。我们揭示这两个因素都在成熟细胞（包括室管膜细胞，中间神经元和运动神经元）的相似队列中表达。我们还显示了脊髓损伤后巢蛋白表达细胞中NFIA和NFIB的强健和广泛表达。总体而言，这些数据为进一步定义NFIA和NFIB在成人脊髓中起什么功能性作用提供了基础。