The Lateral Hypothalamus/Perifornical Area (LH/PFA) has been shown to be involved with the hypercapnic ventilatory response, in a state-dependent manner. We have demonstrated that purinergic signaling through ATP in the LH/PFA has an excitatory effect in ventilatory response to CO2 in awake rats in the dark phase of the diurnal cycle, but it is unknown whether the ATP metabolite adenosine, acting in the LH/PFA, modulates the ventilatory responses to hypercapnia. Here, we studied the effects of the microdialysis of adenosine (A1/A2 adenosine receptors agonist; 17 mM) and an A1 receptor antagonist (DPCPX; 0.1 mM) into the LH/PFA of conscious rats on ventilation in room air and in 7% CO2 during the light and the dark phases of the diurnal cycle. The microdialysis of adenosine and DPCPX caused no change in the CO2 ventilatory responses of rats during wakefulness or NREM sleep in either the dark or light period. Our data suggest that adenosine in the LH/PFA does not contribute to the hypercapnic ventilatory response in conscious rats.

中文翻译：

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外侧下丘脑/穹窿周围区域的腺苷不参与 CO2 化学反射。

外侧下丘脑/眶周区 (LH/PFA) 已被证明以状态依赖的方式参与高碳酸血症通气反应。我们已经证明，在昼夜循环的黑暗阶段，通过 LH/PFA 中 ATP 的嘌呤能信号传导对清醒大鼠对 CO2 的通气反应具有兴奋作用，但尚不清楚 ATP 代谢物腺苷是否在 LH/PFA 中起作用，调节对高碳酸血症的通气反应。在这里，我们研究了腺苷（A1/A2 腺苷受体激动剂；17 mM）和 A1 受体拮抗剂（DPCPX；0.1 mM）微透析进入清醒大鼠的 LH/PFA 对室内空气和 7% 的通风的影响昼夜循环的明暗阶段的二氧化碳。腺苷和 DPCPX 的微透析导致大鼠在清醒或 NREM 睡眠期间在黑暗或光明时期的 CO2 通气反应没有变化。我们的数据表明，LH/PFA 中的腺苷对清醒大鼠的高碳酸血症通气反应没有贡献。