当前位置: X-MOL 学术Domest. Anim. Endocrinol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Possible roles of parathyroid hormone, 1.25(OH)2D3, and fibroblast growth factor 23 on genes controlling calcium metabolism across different tissues of the laying hen.
Domestic Animal Endocrinology ( IF 2.1 ) Pub Date : 2019-10-23 , DOI: 10.1016/j.domaniend.2019.106407
A Gloux 1 , N Le Roy 1 , J Ezagal 1 , N Même 1 , C Hennequet-Antier 1 , M L Piketty 2 , D Prié 2 , G Benzoni 3 , J Gautron 1 , Y Nys 1 , A Narcy 1 , M J Duclos 1
Affiliation  

This study provides an integrative description of candidate gene expression across tissues involved in calcium (Ca) metabolism during the egg laying cycle, using the well-defined model of Ca supply as fine or coarse particles of calcium carbonate (CaCO3). Plasma and tissue samples were collected from hens at the peak of laying at 0 to 1, 9 to 10, and 18 to 19 h postovulation (PO). After mRNA preparation from the parathyroid gland, medullary bone, liver, kidney, duodenum, and jejunum, gene expressions were quantified using RT-qPCR. The highest levels of parathyroid hormone (PTH) mRNA in the parathyroid gland (P < 0.05), and of the active form of vitamin D3 1.25(OH)2D3 in the plasma (P < 0.01) were observed at 18 to 19 h PO. During this active phase of eggshell formation, bone resorption was attested to high levels of plasma inorganic phosphorus (iP) and the receptor activation of nuclear factor-κB expression in the bone (P < 0.001 and P < 0.05, respectively). At this stage, 5 genes of the transcellular and the paracellular Ca absorption pathways in the intestine (P < 0.05) and the Ca channel transient receptor potential cation channel subfamily V member 5 (P < 0.05), involved in its reabsorption in the kidney, were overexpressed. At 0 to 1 h PO during the subsequent daylight period, 2 candidates of the transcellular and the paracellular Ca pathways (P < 0.05) remained at high levels in the intestine, while calbindin D 28K expression was the highest in the kidney (P < 0.05). As PTH mRNA and 1.25(OH)2D3 were low, bone accretion was likely active at this stage. The phosphaturic hormone fibroblast growth factor 23 (FGF23) was overexpressed at 18 to 19 h PO (P < 0.05) in the bone when plasma iP was high, which suggested a role in the subsequent reduction of P reabsorption in the kidney, as attested to the decreased expression of P cotransporters, leading to iP clearance from the plasma at 0 to 1 h PO (P < 0.05). The low levels of 1.25(OH)2D3 at this stage coincided with increased expression of the 24-hydroxylase gene in the kidney (P < 0.05). In hens fed fine particles of CaCO3, higher plasma levels of 1,25(OH)2D3 and higher expression of several genes involved in bone turnover reflected a stronger challenge to Ca homeostasis. Altogether, these data support the hypothesis that FGF23 could drive vitamin D metabolism in the laying hen, as previously documented in other species and explain the tight link between P and Ca metabolisms.



中文翻译:

甲状旁腺激素,1.25(OH)2D3和成纤维细胞生长因子23在蛋鸡不同组织中控制钙代谢的基因上的可能作用。

这项研究使用精确定义的Ca供给模型,即碳酸钙的细颗粒或粗颗粒(CaCO 3),提供了在产卵周期中参与钙(Ca)代谢的组织中候选基因表达的完整描述。在排卵后0至1、9至10和18至19 h的产蛋高峰时从母鸡收集血浆和组织样本。从甲状旁腺,髓质骨,肝,肾,十二指肠和空肠制备mRNA后,使用RT-qPCR定量基因表达。甲状旁腺中甲状旁腺激素(PTH)mRNA的最高水平(P <0.05),血浆中维生素D 3 1.25(OH)2 D 3的活性形式最高(P<0.01)在PO的18至19 h观察到。在蛋壳形成的这个活跃阶段,骨吸收被证明具有高水平的血浆无机磷(i P)和骨骼中核因子-κB表达的受体激活(分别为P <0.001和P <0.05)。在此阶段,肠中跨细胞和旁细胞Ca吸收途径的5个基因(P <0.05)和Ca通道瞬态受体电位阳离子通道亚家族V成员5(P <0.05),涉及其在肾脏中的重吸收,被过度表达。在随后的白天,PO在0至1 h时,有2个候选的跨细胞和旁细胞Ca途径(P<0.05)在肠道中保持高水平,而钙结合蛋白D 28K在肾脏中的表达最高(P <0.05)。由于PTH mRNA和1.25(OH)2 D 3较低,因此在此阶段骨增生很可能活跃。当血浆i P较高时,骨中的磷酸性激素成纤维细胞生长因子23(FGF23)在骨中过表达18至19 h PO(P <0.05),这证明了其在肾中随后减少P重吸收的作用。 P共转运蛋白的表达降低,导致在0至1 h PO时血浆中i P清除(P <0.05)。1.25(OH)2 D 3的含量低在此阶段,与肾脏中24-羟化酶基因表达的增加相吻合(P <0.05)。在饲喂细颗粒的CaCO 3的母鸡中,较高的血浆水平1,25(OH)2 D 3和参与骨骼更新的几个基因的较高表达反映了对Ca动态平衡的更大挑战。总而言之,这些数据支持如下假设:FGF23可以驱动蛋鸡中的维生素D代谢,如先前在其他物种中所记录的那样,并解释了P和Ca代谢之间的紧密联系。

更新日期:2019-10-23
down
wechat
bug