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Myo1e modulates the recruitment of activated B cells to inguinal lymph nodes.
Journal of Cell Science ( IF 4 ) Pub Date : 2020-03-02 , DOI: 10.1242/jcs.235275
Daniel A Girón-Pérez 1 , Eduardo Vadillo 1 , Michael Schnoor 1 , Leopoldo Santos-Argumedo 2
Affiliation  

The inclusion of lymphocytes in high endothelial venules and their migration to the lymph nodes are critical steps in the immune response. Cell migration is regulated by the actin cytoskeleton and myosins. Myo1e is a long-tailed class I myosin and is highly expressed in B cells, which have not been studied in the context of cell migration. By using intravital microscopy in an in vivo model and performing in vitro experiments, we studied the relevance of Myo1e for the adhesion and inclusion of activated B cells in high endothelial venules. We observed reduced expression of integrins and F-actin in the membrane protrusions of B lymphocytes, which might be explained by deficiencies in vesicular trafficking. Interestingly, the lack of Myo1e reduced the phosphorylation of focal adhesion kinase (FAK; also known as PTK2), AKT (also known as AKT1) and RAC-1, disturbing the FAK-PI3K-RAC-1 signaling pathway. Taken together, our results indicate a critical role of Myo1e in the mechanism of B-cell adhesion and migration.

中文翻译:

Myo1e调节激活的B细胞向腹股沟淋巴结的募集。

淋巴细胞在高内皮小静脉中的包容和向淋巴结的迁移是免疫反应中的关键步骤。细胞迁移受肌动蛋白细胞骨架和肌球蛋白的调节。Myo1e是长尾类I肌球蛋白,在B细胞中高度表达,尚未在细胞迁移的背景下进行研究。通过在体内模型中使用活体显微镜检查并进行体外实验,我们研究了Myo1e与高内皮小静脉中活化B细胞的黏附和包涵的相关性。我们观察到B淋巴细胞膜突起中整合素和F-肌动蛋白的表达减少,这可能是由于水泡运输不足所致。有趣的是,缺乏Myo1e会减少粘着斑激酶(FAK;也称为PTK2)的磷酸化,AKT(也称为AKT1)和RAC-1,干扰了FAK-PI3K-RAC-1信号通路。两者合计,我们的结果表明Myo1e在B细胞粘附和迁移机制中的关键作用。
更新日期:2020-03-16
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