Myosins make up a large super family of motor proteins responsible for actin-based motility in most eukaryotic cells. Myosin VIIA is essential for the development and function of sensory hair cells in the inner ear. The role of Myosin VIIA in the development of cochleovestibular ganglion (CVG) neurons in the mouse is largely unknown. Neurons of the CVG innervate sensory hair cells of the cochlea and vestibular organs to transmit hearing and balance information respectively to the brain. The aim of this study was to characterize the expression of MYOSIN VIIA in the CVG of mouse embryos. Spatiotemporal expression of MYOSIN VIIA was characterized in embryonic (E) mouse inner ear neurons from E9.5 to postnatal (P) day 0. At early stages, when otic neurons begin to delaminate to form the CVG, MYOSIN VIIA was co-expressed with TuJ1, ISLET1 and NEUROD in the otic epithelium and CVG. When CVG neurons were migrating and exiting mitosis, MYSOSIN VIIA was downregulated in a subset of neurons, which were NEUROD-negative and GATA3-positive. After segregation of the CVG, MYOSIN VIIA was observed in a subset of vestibular neurons marked by TUJ1 and absent in cochlear neurons, marked by GATA3. The differential expression of MYOSIN VIIA may indicate a role in inner ear neuron migration and specific labeling of vestibular neurons.

肌球蛋白构成了一个大的运动蛋白超家族，负责大多数真核细胞中基于肌动蛋白的运动。肌球蛋白 VIIA对内耳感觉毛细胞的发育和功能至关重要。肌球蛋白VIIA的作用小鼠耳蜗前庭神经节 (CVG) 神经元的发育过程在很大程度上是未知的。CVG 的神经元支配耳蜗和前庭器官的感觉毛细胞，分别向大脑传递听觉和平衡信息。本研究的目的是表征 MYOSIN VIIA 在小鼠胚胎 CVG 中的表达。MYOSIN VIIA 在胚胎 (E) 小鼠内耳神经元中的时空表达特征是从 E9.5 到出生后 (P) 第 0 天。在早期阶段，当耳神经元开始分层形成 CVG 时，MYOSIN VIIA 与耳上皮和 CVG 中的 TuJ1、ISLET1 和 NEUROD。当 CVG 神经元迁移并退出有丝分裂时，MYSOSIN VIIA 在神经元亚组中下调，这些神经元为 NEUROD 阴性和 GATA3 阳性。分离 CVG 后，在由 TUJ1 标记的前庭神经元子集中观察到肌球蛋白 VIIA，而在以 GATA3 标记的耳蜗神经元中不存在。MYOSIN VIIA 的差异表达可能表明在内耳神经元迁移和前庭神经元的特异性标记中起作用。