Pregnane X receptor (PXR) as a ligand dependent transcription factor, is capable of regulating gene expression of cytochromes P450 and transporters involved in xenobiotic/drug metabolism and elimination. Due to the species differences in the regulatory specificity of PXR, gene regulation should not be extrapolated from mammal to fish without research data.

The aim of present study was to investigate the effect of 27 natural products on PXR, CYP3A30 and MDR1 genes in channel catfish (Ietalurus punetaus) kidney cells (CC-K).

The results showed that bisdemethoxycurcumin, glycyrrhetnic acid, rotenone, artemisinin, dihydroartemisinin, ligustilide and matrine strongly induced the mRNA levels of PXR. Additionally, the up-regulation of CYP3A30 gene ran parallel with PXR gene after the treatment of demethoxycurcumin, glycyrrhetnic acid, artemisinin, matrine, baicalein, schisantherin A, ligustilide, and dihydroartemisinin. Moreover, we found that natural products schisandrin A, schisandrin B, schisandrol A, and schisandrol B significantly up-regulated the mRNA level of MDR1 gene.

Our work with a view to provide experimental data support for further research, which will make for the rational application of natural products in channel catfish, such as to avoid adverse herb-drug interactions or accelerating the residue elimination of chemical medicine.

孕烷X受体（PXR）作为配体依赖性转录因子，能够调节细胞色素P450的基因表达以及参与异源/药物代谢和消除的转运蛋白。由于PXR调控特异性的物种差异，如果没有研究数据，则不应将基因调控从哺乳动物推算到鱼类。

本研究的目的是研究27种天然产物对channel鱼（Ietalurus punetaus）肾细胞（CC-K）中PXR，CYP3A30和MDR1基因的影响。

结果表明，双去甲氧基姜黄素，甘草次酸，鱼藤酮，青蒿素，二氢青蒿素，li本内酯和苦参碱强烈诱导PXR的mRNA水平。此外，在去甲氧基姜黄素，甘草次酸，青蒿素，苦参碱，黄ical素，schisantherin A，li本草酯和二氢青蒿素处理后，CYP3A30基因的上调与PXR基因平行。此外，我们发现天然产物五味子素A，五味子素B，五味子素A和五味子素B显着上调了MDR1基因的mRNA水平。

我们的工作旨在为进一步的研究提供实验数据支持，这将使天然产物合理地用于channel鱼中，例如避免不良的药草相互作用或加速化学药物的残留消除。