1. Functional microRNA (miRNA) screening for abdominal fat tissue with different dietary vitamin E (VE) levels was performed to reveal miRNAs, genes and metabolic pathways involved in abdominal fat deposition in broilers.

2. A total of 240, one-day-old healthy female chicks were randomly allocated into five dietary treatments containing either 0, 20, 50, 75 or 100 IU DL-α-tocopherol acetate. The sequencing of miRNAs from abdominal fat tissues was performed. The target genes of miRNAs were predicted and enrichment analysis for these genes was performed. Diets supplemented with 50 IU VE significantly diminished abdominal fat deposition in broilers at day 35 of age.

3. A total of 29 miRNAs were differentially expressed between control and 50 IU VE treatment. Ten of the 23 target genes were enriched in four signalling pathways: tight junction, SNARE interactions in vesicular transport, regulation of autophagy and proteasome.

4. This study identified miRNA, target genes and pathways in dietary VE treatment for broilers, providing new insights into the miRNA regulation of abdominal fat deposition in broilers.

1. 对不同日粮维生素 E (VE) 水平的腹部脂肪组织进行功能性 microRNA (miRNA) 筛选，以揭示参与肉鸡腹部脂肪沉积的 miRNA、基因和代谢途径。

2. 将总共 240 只 1 日龄健康雌性小鸡随机分配到含有 0、20、50、75 或 100 IU DL-α-生育酚乙酸酯的五种饮食处理中。对来自腹部脂肪组织的 miRNA 进行测序。预测miRNA的靶基因并进行这些基因的富集分析。添加 50 IU VE 的日粮显着减少了 35 日龄肉鸡的腹部脂肪沉积。

3. 对照和 50 IU VE 处理共有 29 个 miRNAs 差异表达。23 个靶基因中有 10 个富含四种信号通路：紧密连接、囊泡运输中的 SNARE 相互作用、自噬和蛋白酶体的调节。

4. 本研究确定了肉鸡日粮VE处理中的miRNA、靶基因和通路，为肉鸡腹部脂肪沉积的miRNA调控提供了新的见解。