Introduction: Visceral leishmaniasis (VL) is a life-threatening infection remaining as one of the most neglected tropical diseases around the world. Despite scientific advances, an accurate diagnosis of VL remains a challenge. Loop-mediated isothermal amplification (LAMP) has emerged as a promising diagnostic tool with the possibility of becoming a point-of-care test to guide VL diagnosis and treatment.Areas covered: We conducted a systematic review assessing LAMP systems for diagnosing VL from 2000 to 2019. We performed structured searches in PubMed, LILACS, Scopus, and Web of Science without language restriction. Two reviewers screened articles, completed the data extraction and assessment of the risk of bias. A qualitative summary of the included studies was performed.Expert opinion: LAMP could be used as a screening test for VL diagnosis, so tissue aspiration could be performed only for those who are LAMP negative. We recommend more studies about the performance of the Loopamp™ Leishmania Detection kit and the Brazilian LAMP assay. Thus, we expect in the future the constitution of an international consortium to share experiences, projects, and other LAMP approaches mainly among researchers and institutions located within VL endemic countries.

中文翻译：

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用于诊断内脏利什曼病（黑热病）的环介导等温扩增方法 - 系统评价。

简介：内脏利什曼病 (VL) 是一种威胁生命的感染，仍然是世界上最被忽视的热带疾病之一。尽管科学取得了进步，但准确诊断 VL 仍然是一个挑战。环介导的等温扩增 (LAMP) 已成为一种很有前途的诊断工具，有可能成为指导 VL 诊断和治疗的即时检验。涵盖的领域：我们从 2000 年开始对 LAMP 系统进行系统评价以诊断 VL到 2019 年。我们在 PubMed、LILACS、Scopus 和 Web of Science 中进行了结构化搜索，没有语言限制。两名审稿人筛选文章，完成数据提取和偏倚风险评估。对纳入的研究进行定性总结。 专家意见：LAMP 可作为 VL 诊断的筛选试验，因此只能对 LAMP 阴性的患者进行组织抽吸。我们建议对 Loopamp™ 利什曼原虫检测试剂盒和巴西 LAMP 检测的性能进行更多研究。因此，我们预计未来将组建一个国际联盟，主要在 VL 流行国家的研究人员和机构之间分享经验、项目和其他 LAMP 方法。