Antidepressant drugs are one of the most widely used medicines for treating major depressive disorders for long time periods. Oral fluid (OF) testing offers an easy and non-invasive sample collection. Detection of antidepressants in OF is important in clinical and forensic settings, such as therapeutic drug monitoring and roadside testing for driving under influence. We developed and validated a comprehensive liquid chromatography–tandem mass spectrometry method for 18 antidepressants (amitriptyline, bupropion, citalopram, clomipramine, cyclobenzaprine, desipramine, desvenlafaxine, doxepin, duloxetine, fluoxetine, imipramine, mirtazapine, nortriptyline, paroxetine, sertraline, trazodone, trimipramine, venlafaxine) in oral fluid collected by Quantisal® oral collection devices. One-half milliliter of Quantisal® OF (125 μL of neat OF) was submitted to solid-phase extraction. The chromatographic separation was performed employing a biphenyl column in gradient mode with a total run time of 5 min. The MS detection was achieved by multiple-reaction monitoring with two transitions per compound. The range for linearity of all analytes was from 10 to 1,000 ng/mL, with a limit of detection of 10 ng/mL. Intra and inter-day accuracy and precision (n = 15) were all within acceptable limits, ±20% error and ±15% relative standard deviation. Analyte recovery at 400 ng/mL concentration (n = 15) ranged from 91 to 129%. Matrix effect ranged from 73.7 to 157%. The internal proficiency test detected all antidepressants with accuracy ranging from 83.1 to 112.1%. The authentic patient sample showed a percentage difference compared to the previously calculated concentration of 86.3–111%. This method provides for the rapid detection of 18 antidepressants and metabolites in OF, which is readily applicable to a routine laboratory.

中文翻译：

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使用SPE和LC-MS-MS开发并验证快速准确的方法来定量口服液样品中的18种抗抑郁药。

抗抑郁药是长期用于治疗严重抑郁症的最广泛使用的药物之一。口服液（OF）测试可轻松，无创地收集样品。在临床和法医环境中检测OF中的抗抑郁药非常重要，例如治疗性药物监测和路边行驶中的路测。我们开发并验证了一种适用于18种抗抑郁药（阿米替林，安非他酮，西酞普兰，氯米帕明，环苯扎林，地昔帕明，去甲文拉法辛，多西平，度洛西汀，氟西汀，亚米拉明，米氮平，曲美托拉汀，去甲替林， （venlafaxine），由Quantisal®口腔收集装置收集。二分之一的Quantisal®OF（125μL的纯OF）进行固相萃取。使用联苯柱以梯度模式进行色谱分离，总运行时间为5分钟。MS检测是通过多反应监测实现的，每个化合物有两个跃迁。所有分析物的线性范围为10到1,000 ng / mL，检测极限为10 ng / mL。日内和日间精度和精确度（n  = 15）均在可接受的范围内，误差为±20％，相对标准偏差为±15％。在400 ng / mL浓度（n  = 15）下，分析物的回收率为91％至129％。基质效应从73.7至157％不等。内部能力测试检测到所有抗抑郁药，准确率从83.1％到112.1％。与先前计算的浓度86.3–111％相比，真实的患者样品显示出百分比差异。该方法可快速检测OF中的18种抗抑郁药和代谢物，可轻松应用于常规实验室。