LC-MS/MS-based proteomics coupled with an online bioinformatics platform was under evaluation for applicability to toxicological pathways evaluation at low cytotoxic concentration (LC₁₀) of silver nanoparticles (AgNP) and ionic silver in human carcinoma cells after 48 h of exposure. Significantly, differentially-expressed proteins (One-way ANOVA, p < 0.05) with more than 4-fold compared to the control were subjected to functional pathway analysis by STITCH. SOTA clustering indicated a similarity of the protein expression between AgNP and the control group. We established a resemblance of proteins in the cell cycle pathway affected by both Ag substances. The differences in the toxicological pathways from AgNO₃ were involved in the cellular organization and metabolic process of macromolecules, while the nucleic acid metabolic process was altered by AgNP. The present study supported the practicability of LC-MS/MS-based proteomics coupled with STITCH for the identification of toxicological pathways in both silvers. We appraised this platform technology to be promising and powerful for a toxicological screening of other new substances.

基于 LC-MS/MS 的蛋白质组学与在线生物信息学平台相结合，正在评估银纳米颗粒 (AgNP) 和银离子暴露 48 小时后在低细胞毒性浓度 (LC 10 ) 下对人类癌细胞毒理学途径评估的适用性_。值得注意的是，与对照相比，差异表达蛋白（单向方差分析，p < 0.05）超过 4 倍，并通过 STITCH 进行功能通路分析。SOTA 聚类表明 AgNP 和对照组之间的蛋白质表达具有相似性。我们确定了受两种银物质影响的细胞周期途径中蛋白质的相似性。_{AgNO 3}毒理学途径的差异涉及细胞组织和大分子的代谢过程，而AgNP改变了核酸代谢过程。本研究支持基于 LC-MS/MS 的蛋白质组学与 STITCH 结合用于鉴定两种银的毒理学途径的实用性。我们认为该平台技术对于其他新物质的毒理学筛选来说是有前途且强大的。