Analytical Sciences ( IF 1.6 ) Pub Date : 2020-09-10 , DOI: 10.2116/analsci.19p475 Ze Wang 1 , Bin Dong 1 , Xiaoqian Cui 2 , Qian Fan 3 , Yanfu Huan 1 , Hongyan Shan 1 , Guodong Feng 1 , Qiang Fei 1
In this study, core-shell Au@Pt nanoparticles (Au@Pt NPs) with peroxidase catalytic activity were synthesized by the seed-mediated method, and were used to catalyze the reaction of luminol–H2O2 to enhance the chemiluminescence (CL) intensity. It was found that thiocyanate (SCN−) can effectively inhibit the catalytic activity of Au@Pt NPs. Based on this phenomenon, a method to detect SCN− by using the Au@Pt NPs-catalytic luminol–H2O2 CL system was established, which has an ultra-low detection limit and an ultra-wide linear range, as well as the advantages of being simple and having low-cost and convenient operation. The research mechanism indicated that SCN− could be adsorbed on the surface of Au@Pt NPs and occupies the active sites of Pt nanostructures, which led to a decrease in the amount of Pt0 and a loss of the excellent catalytic activity of Au@Pt NPs. After optimizing the experimental conditions, this assay for detecting SCN− exhibited a good linear range from 5 to 180 nM, and the low detection limit was 2.9 nM. In addition, this approach has been successfully applied to the detection of SCN− in tap-water samples, which has practical application value and embodies good development prospects.
Fullsize Image中文翻译:
核壳 Au@Pt 纳米颗粒催化鲁米诺化学发光用于硫氰酸盐的灵敏检测。
本研究采用种子介导的方法合成了具有过氧化物酶催化活性的核壳型Au@Pt纳米粒子(Au@Pt NPs),并用于催化鲁米诺-H 2 O 2反应以增强化学发光(CL ) 强度。发现硫氰酸盐(SCN -)可以有效抑制Au@Pt NPs的催化活性。基于这一现象,建立了一种利用Au@Pt NPs-催化鲁米诺-H 2 O 2 CL体系检测SCN -的方法,该方法具有超低的检测限和超宽的线性范围,以及具有简单、成本低、操作方便的优点。研究机制表明,SCN -可以吸附在Au@Pt NPs的表面并占据Pt纳米结构的活性位点,导致Pt 0的量减少和Au@Pt NPs优异的催化活性丧失。优化实验条件后,该检测SCN-的方法在5-180 nM范围内表现出良好的线性范围,检测下限为2.9 nM。此外,该方法已成功应用于自来水样品中SCN-的检测,具有实际应用价值,体现了良好的发展前景。
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