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The efficacy of povidone-iodine, hydrogen peroxide and a chemical multipurpose contact lens care system against Pseudomonas aeruginosa on various lens case surfaces.
Contact Lens & Anterior Eye ( IF 3.2 ) Pub Date : 2020-02-27 , DOI: 10.1016/j.clae.2020.02.012
Katsuhide Yamasaki 1 , Yohei Mizuno 1 , Yoshiyuki Kitamura 1 , David J McCanna 2 , William Ngo 2 , Lyndon W Jones 2
Affiliation  

Purpose

To determine the antimicrobial efficacy of a povidone-iodine system (PVP-I; cleadew, OPHTECS Corporation, Kobe, Japan), a peroxide system (AOSEPT Plus with HydraGlyde, Alcon, Fort Worth, TX), and a chemical multipurpose system (renu fresh, Bausch & Lomb, Rochester, NY) on contact lens case surfaces that are both in contact and not in contact with the solutions during lens disinfection.

Methods

The surfaces of the inner walls, underside of the lid, and lens holder (if applicable) of the cases were inoculated with P. aeruginosa ATCC 27853. The cases were disinfected with the solutions as per their manufacturer instructions. After disinfection, the inoculated surfaces were swabbed and the amount of surviving P. aeruginosa was determined. Following this experiment, separate cases were inoculated and disinfected as before. This time the cases were agitated after recommended disinfection time and the amount of P. aeruginosa in the disinfecting solution was quantified immediately, and again after resting for 7 days. Experiments were conducted in triplicate (n = 3).

Results

Units are expressed in log CFU. All three solutions significantly reduced P. aeruginosa on direct-contact surfaces (all p < 0.039). On non-contact surfaces, the reduction of P. aeruginosa in the PVP-I system (pre-disinfection: 6.8 ± 0.5, post-disinfection: 1.0 ± 0.0; p < 0.001) was significant, but not for the hydrogen peroxide system (pre-disinfection: 6.3 ± 0.6, post: 5.5 ± 0.5; p = 0.194) and the chemical multipurpose system (pre-disinfection: 6.6 ± 0.1, post-disinfection: 5.6 ± 0.8; p = 0.336). After 7 days post-disinfection, no P. aeruginosa regrowth was observed in the PVP-I system (Day 1: 1.0 ± 0.0, Day 7: 1.0 ± 0.0; p = 1) and the chemical multipurpose system (Day 1: 4.2 ± 0.2, Day 7: 1.8 ± 0.9; p = 0.012), however regrowth was observed in the hydrogen peroxide system (Day 1: 3.4 ± 0.6, Day 7: 6.1 ± 0.4; p = 0.003).

Conclusion

The PVP-I system was more effective against P. aeruginosa on non-contact surfaces than the hydrogen peroxide system or the chemical multipurpose system and is capable of inhibiting regrowth of P. aeruginosa for at least 7 days post-disinfection.



中文翻译:

聚维酮碘、过氧化氢和化学多用途隐形眼镜护理系统对各种镜片盒表面上的铜绿假单胞菌的功效。

目的

确定聚维酮碘系统(PVP-I;cleadew,OPHTECS Corporation,日本神户)、过氧化物系统(AOSEPT Plus with HydraGlyde,Alcon,Fort Worth,TX)和化学多用途系统(renu新鲜,博士伦,罗彻斯特,纽约)在隐形眼镜盒表面上,在镜片消毒过程中接触和不接触溶液。

方法

铜绿假单胞菌ATCC 27853接种盒的内壁、盖子下侧和透镜架(如果适用)。按照制造商的说明用溶液对盒进行消毒。消毒后,对接种的表面进行擦拭并测定存活的铜绿假单胞菌的数量。在这个实验之后,像以前一样对不同的病例进行接种和消毒。这次在推荐的消毒时间后搅拌病例,立即对消毒液中铜绿假单胞菌的数量进行定量,静置7天后再次进行定量。实验一式三份进行(n = 3)。

结果

单位以 log CFU 表示。所有三种溶液都显着减少了直接接触表面上的铜绿假单胞菌(所有 p < 0.039)。在非接触表面上,PVP-I 系统(消毒前:6.8 ± 0.5,消毒后:1.0 ± 0.0;p < 0.001)中铜绿假单胞菌的减少是显着的,但对于过氧化氢系统则不然(消毒前:6.3 ± 0.6,后:5.5 ± 0.5;p = 0.194)和化学多用途系统(消毒前:6.6 ± 0.1,消毒后:5.6 ± 0.8;p = 0.336)。消毒 7 天后,没有铜绿假单胞菌 在 PVP-I 系统(第 1 天:1.0 ± 0.0,第 7 天:1.0 ± 0.0;p = 1)和化学多用途系统(第 1 天:4.2 ± 0.2,第 7 天:1.8 ± 0.9;p = 0.012),但是在过氧化氢系统中观察到再生(第 1 天:3.4 ± 0.6,第 7 天:6.1 ± 0.4;p = 0.003)。

结论

PVP-I 系统对非接触表面上的铜绿假单胞菌比过氧化氢系统或化学多用途系统更有效,并且能够在消毒后至少 7 天内抑制铜绿假单胞菌的再生。

更新日期:2020-02-27
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