ABSTRACT

The present investigation is focused on development of aerobic biofilm on tire-derived rubber (TDR) media and then evaluation of such system for bioremediation of Methylene blue (MB) dye for 9 weeks. After 9 weeks of operation, the COD, BOD, ammonia and color values have been declined by 89.2%, 98.3%, 99.61% and 99.81%, respectively, While SEM-EDX results showed a variance in weight percent of various elements in TDR without biofilm i.e. raw TDR media, as well as in the 1st and 9th-week samples. Moreover, fine and strong peaks were observed in both the MB simulated wastewater and 9th week TDR samples at 1190, 1300, 1400, 1450, 1500 and 1618 cm⁻¹ respectively by Raman Spectroscopic analysis. Further, FTIR analysis was performed for the MB simulated wastewater, and absorbance peaks ranging from 1591 to 1363 cm^-1 and 3410 cm⁻¹ were observed in all the samples with different intensities. To assess the biodeterioration of the TDR media, ATR was performed for the raw, 1st, 2nd and 9th week TDR media samples and in the raw TDR, two important bands, 842 and 2962 cm⁻¹ were noticed representing –CH = CH and –CH₃. A clear variation of bands and peak intensities were observed in different support media samples. The results indicate that TDR media is a resilient, chemically resistant material and could be employed for the biofilm growth for biological treatment of textile dye wastewater.

摘要

本研究的重点是在轮胎衍生橡胶 (TDR) 介质上开发有氧生物膜，然后评估这种系统对亚甲蓝 (MB) 染料的生物修复 9 周。运行 9 周后，COD、BOD、氨和色值分别下降了 89.2%、98.3%、99.61% 和 99.81%，而 SEM-EDX 结果显示 TDR 中各种元素的重量百分比变化生物膜，即原始 TDR 培养基，以及第 1 周和第 9 周的样本。此外，通过拉曼光谱分析，分别在 1190、1300、1400、1450、1500和 1618 cm ^-1处的 MB 模拟废水和第 9 周 TDR 样品中均观察到精细和强峰。此外，对 MB 模拟废水进行了 FTIR 分析，吸收峰范围从 1591 到 1363 cm^-1和 3410 cm ^-1在所有具有不同强度的样品中观察到。为了评估 TDR 培养基的生物降解，对原始、第 1、第 2 和第 9 周 TDR 培养基样品进行了 ATR，在原始 TDR 中，注意到两个重要的条带，842 和 2962 cm ^-1代表 –CH = CH 和 –通道₃。在不同的支持介质样品中观察到条带和峰强度的明显变化。结果表明，TDR 介质是一种有弹性、耐化学腐蚀的材料，可用于纺织染料废水生物处理的生物膜生长。