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Effects of Eimeria tenella infection on the barrier damage and microbiota diversity of chicken cecum.
Poultry Science ( IF 4.4 ) Pub Date : 2020-01-22 , DOI: 10.1016/j.psj.2019.10.073
Bian-Hua Zhou 1 , Liu-Shu Jia 1 , Shan-Shan Wei 1 , Hai-Yan Ding 1 , Jing-Yun Yang 1 , Hong-Wei Wang 1
Affiliation  

The symbiosis of host and intestinal microbiota constitutes a microecosystem and plays an important role in maintaining intestinal homeostasis and regulating the host's immune system. Eimeria tenella, an obligate intracellular apicomplexan parasite, can cause coccidiosis, a serious intestinal disease. In this study, the effects of E. tenella infection on development parameters (villus height, crypt depth, mucosa thickness, muscularis thickness, and serosa thickness) and microbiota in chicken cecum were investigated. Fourteen-day-old male Hy-Line Variety Brown layer chickens were inoculated with sporulated oocysts of E. tenella. Cecal tissues were collected 7 d after inoculation. Relative density of goblet cells and glycoproteins were determined by Alcian blue periodic acid-Schiff staining and periodic acid-Schiff staining, respectively. Intestinal development parameters were also evaluated. Cecal contents were extracted, and the composition of cecal microflora was examined by Illumine sequencing in the V3-V4 region of the 16S rRNA gene. Results indicated that E. tenella infection destroyed the structure of cecal tissue and reduced the relative density of goblet cells and glycoproteins. Sequencing analysis indicated that E. tenella infection altered the diversity and composition of cecal microbiota. The populations of Proteobacteria, Enterococcus, Incertae, and Escherichia-Shigella decreased, and those of Bacteroidales and Rikenella significantly increased in the infected group compared with those in the control group. Hence, the pathological damage caused by E. tenella infection is associated with cecal microbiota dysbiosis, and this finding may be used to develop an alternative measure for alleviating the effect of coccidiosis on the poultry industry.

中文翻译:

艾美球虫感染对鸡盲肠屏障损伤和微生物群多样性的影响。

宿主与肠道菌群的共生构成了一个微生态系统,在维持肠道稳态和调节宿主免疫系统中起着重要作用。艾美球虫(Eimeria tenella)是一种专性的细胞内apicomplexan寄生虫,可引起球虫病,一种严重的肠道疾病。在这项研究中,研究了大肠杆菌盲肠感染对鸡盲肠发育参数(绒毛高度,隐窝深度,粘膜厚度,肌层厚度和浆膜厚度)和微生物群的影响。将十四日龄的雄性Hy-Line变种棕色蛋鸡接种有孢子的大肠埃希氏菌卵囊。接种后7天收集盲肠组织。杯状细胞和糖蛋白的相对密度分别通过阿尔辛蓝高碘酸-希夫染色和高碘酸-希夫染色确定。还评估了肠道发育参数。提取盲肠内容物,并通过Illumine测序在16S rRNA基因的V3-V4区域中检查盲肠菌群的组成。结果表明,E。tenella感染破坏了盲肠组织的结构,并降低了杯状细胞和糖蛋白的相对密度。测序分析表明,大肠杆菌感染改变了盲肠菌群的多样性和组成。与对照组相比,感染组中的变形杆菌,肠球菌,不动杆菌和大肠埃希氏菌的种群减少,而杆菌科和里克氏菌的种群显着增加。因此,由大肠杆菌感染引起的病理损害与盲肠微生物群失调有关,
更新日期:2020-01-22
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