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Behavioral and physiological effects of Viola spp. cyclotides on Myzus persicae (Sulz.).
Journal of Insect Physiology ( IF 2.2 ) Pub Date : 2020-02-11 , DOI: 10.1016/j.jinsphys.2020.104025
Katarzyna Dancewicz 1 , Blazej Slazak 2 , Małgorzata Kiełkiewicz 3 , Małgorzata Kapusta 4 , Jerzy Bohdanowicz 4 , Beata Gabryś 1
Affiliation  

Cyclotides are defense peptides produced by several plant families. Viola spp. (Violaceae) produce an array of cyclotides with varying biological activities. The peach potato aphid Myzus persicae (Sulz.) (Hemiptera: Aphididae) is a generalist that feeds on the secondary hosts of over 40 plant families, including Violaceae. The present work aimed to evaluate the activities of cycloviolacins from Viola odorata L. and V. ulignosa Besser (cyO2, cyO3, cyO13, cyO19) against M. persicae. To investigate the peptides' influence on aphid feeding behavior, we used 20% sucrose diets supplemented with cyclotides and measured the effects with electrical penetration graph (EPG) technique. We also applied anti-cyclotide antibodies and immunohistochemistry to track the peptides in the digestive systems of the aphids. Our study shows that cyclotides affect aphid probing and feeding behavior and limit their diet sap uptake. The cycloviolacin cyclotides: cyO13 (100 µM) and cyO19 (50 µM) most strongly impeded aphid ingestion activities when applied in sucrose diet. Sustained ingestion of the diet was blocked by 100 µM cyO13, and no aphid showed ingestion of the diet for longer than 10 min. Cyclotides were detected in the pharynx, in contact with the epipharyngeal gustatory organ, in the stomach (midgut) and upper intestine. The present study shows the deterrent activity of cycloviolacins on M. persicae. This activity may be related to the peptides' effects on epithelial cells and gustatory organs along the aphid digestive system. We demonstrate that cyclotides may play an important role in plant-aphid interactions.

中文翻译:

中提琴的行为和生理效应。桃蚜(Syz。persicae)。

环肽是几种植物家族产生的防御肽。中提琴属 (堇菜科)产生具有不同生物活性的一系列环核苷酸。桃类马铃薯蚜虫Myzus persicae(Sulz。)(半翅目:蚜虫)是通才,以40种植物科(包括堇菜科)的次生寄主为食。目前的工作旨在评估来自中提琴和中叶比索弧菌(cyO2,cyO3,cyO13,cyO19)的环紫胶素对桃蚜的活性。为了研究这些肽对蚜虫摄食行为的影响,我们使用了添加了环糊精的20%蔗糖日粮,并通过电渗透图(EPG)技术测量了效果。我们还应用了抗环肽抗体和免疫组化技术来追踪蚜虫消化系统中的肽段。我们的研究表明,环线虫会影响蚜虫的探测和摄食行为,并限制其饮食中的汁液摄入。当用于蔗糖饮食中时,环紫胶环化物:cyO13(100 µM)和cyO19(50 µM)最强烈地阻碍了蚜虫的摄食活动。饮食的持续摄入被100 µM cyO13阻止,没有蚜虫显示饮食摄入超过10分钟。在咽部,与上咽味觉器官接触,在胃(中肠)和上小肠中发现了环肽。本研究显示了环紫胶对桃蚜的抑制活性。这种活性可能与肽对沿蚜虫消化系统的上皮细胞和味觉器官的作用有关。我们证明,环氧化物可能在植物与蚜虫的相互作用中起重要作用。
更新日期:2020-03-20
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