当前位置:
X-MOL 学术
›
Comp. Immunol. Microbiol. Infect. Dis.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genomic and functional characterization of fecal sample strains of Proteus cibarius carrying two floR antibiotic resistance genes and a multiresistance plasmid-encoded cfr gene.
Comparative Immunology, Microbiology and Infectious Diseases ( IF 2 ) Pub Date : 2020-01-26 , DOI: 10.1016/j.cimid.2020.101427 Tingyuan Zhu 1 , Suzhen Liu 2 , Yuanyuan Ying 1 , Lei Xu 1 , Yabo Liu 1 , Junjie Jin 2 , Jun Ying 1 , Junwan Lu 1 , Xi Lin 1 , Kewei Li 1 , Teng Xu 3 , Qiyu Bao 1 , Peizhen Li 1
Comparative Immunology, Microbiology and Infectious Diseases ( IF 2 ) Pub Date : 2020-01-26 , DOI: 10.1016/j.cimid.2020.101427 Tingyuan Zhu 1 , Suzhen Liu 2 , Yuanyuan Ying 1 , Lei Xu 1 , Yabo Liu 1 , Junjie Jin 2 , Jun Ying 1 , Junwan Lu 1 , Xi Lin 1 , Kewei Li 1 , Teng Xu 3 , Qiyu Bao 1 , Peizhen Li 1
Affiliation
The objective of this study was to investigate the molecular characteristics and horizontal transfer of florfenicol resistance gene-related sequences in Proteus strains isolated from animals. A total of six Proteus strains isolated from three farms between 2015 and 2016 were screened by polymerase chain reaction (PCR) for known florfenicol resistance genes. Proteus cibarius G11, isolated from the fecal material of a goose, was found to harbor both cfr and floR genes. Whole genome sequencing revealed that the strain harbored two copies of the floR gene: one was located on the chromosome and the other was located on a plasmid named pG11-152. Two floR-containing fragments 4028 bp in length were identical and showed transposon-like structures. The cfr gene was found on a plasmid named pG11-51 and flanked by a pair of IS26s. Thus, mobile genetic elements played an important role in floR replication and horizontal resistance gene transfer. Therefore, increasing attention should be paid to monitoring the spread of resistance genes and resistance in real time.
中文翻译:
带有两个floR抗生素抗性基因和一个多抗性质粒编码的cfr基因的变形杆菌粪便样品菌株的基因组和功能表征。
这项研究的目的是调查从动物分离出的变形杆菌菌株中氟苯尼考耐药基因相关序列的分子特征和水平转移。通过聚合酶链反应(PCR)筛选了已知的氟苯尼考抗性基因的2015年至2016年从三个农场分离的六株变形杆菌菌株。从鹅粪便中分离出的变形杆菌Cibarius G11含有cfr和floR基因。全基因组测序表明,该菌株携带了两个拷贝的floR基因:一个位于染色体上,另一个位于名为pG11-152的质粒上。两个长度为4028 bp的含floR的片段相同,并显示出类似转座子的结构。在名为pG11-51的质粒上发现了cfr基因,其侧翼是一对IS26。从而,流动遗传元件在floR复制和水平抗性基因转移中起重要作用。因此,应越来越重视实时监测抗性基因的传播和抗性。
更新日期:2020-01-26
中文翻译:
带有两个floR抗生素抗性基因和一个多抗性质粒编码的cfr基因的变形杆菌粪便样品菌株的基因组和功能表征。
这项研究的目的是调查从动物分离出的变形杆菌菌株中氟苯尼考耐药基因相关序列的分子特征和水平转移。通过聚合酶链反应(PCR)筛选了已知的氟苯尼考抗性基因的2015年至2016年从三个农场分离的六株变形杆菌菌株。从鹅粪便中分离出的变形杆菌Cibarius G11含有cfr和floR基因。全基因组测序表明,该菌株携带了两个拷贝的floR基因:一个位于染色体上,另一个位于名为pG11-152的质粒上。两个长度为4028 bp的含floR的片段相同,并显示出类似转座子的结构。在名为pG11-51的质粒上发现了cfr基因,其侧翼是一对IS26。从而,流动遗传元件在floR复制和水平抗性基因转移中起重要作用。因此,应越来越重视实时监测抗性基因的传播和抗性。