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Local assessment of WC1+ γδ T lymphocyte subset in the different types of lesions associated with bovine paratuberculosis.
Comparative Immunology, Microbiology and Infectious Diseases ( IF 2 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.cimid.2020.101422
Miguel Criado 1 , Julio Benavides 1 , Raquel Vallejo 1 , Noive Arteche 1 , Daniel Gutiérrez 1 , M Carmen Ferreras 1 , Valentín Pérez 1 , José Espinosa 1
Affiliation  

The local expression of WC1+ γδ T lymphocytes subset has been evaluated by immunohistochemical methods at the different types of lesions present in cows naturally infected with Mycobacterium avium subsp. paratuberculosis (Map) and in non-infected control animals. Infected cattle were either in the latent/subclinical (focal lesions) or clinical (diffuse paucibacillary and multibacillary forms) stage of paratuberculosis. To assess the cell distribution, a differential cell count was carried out at the lamina propria, gut-associated lymphoid tissue and submucosa. A significant increase in the number of WC1+ γδ T cells was observed in all the infected animals, regardless of the type of lesion. Cows with focal lesions showed higher number of labeled cells than those with diffuse forms, where no differences were found between the two types. This increase in the number of positively immunolabelled lymphocytes in infected animals was seen in the lamina propria, with higher values in those with focal lesions. While in the lymphoid tissue no differences in the numbers were observed, in animals with focal lesions, WC1+ γδ T cells tended to be located at the periphery of the granulomas. These findings suggest a proinflammatory action of WC1+ γδ T lymphocytes in bovine paratuberculosis, which might play an important role in the containment of the Map-infection in the focal granulomas located in the lymphoid tissue, helping to prevent the progression toward diffuse forms responsible for the clinical signs.

中文翻译:

在与牛副结核相关的不同类型病变中对WC1 +γδT淋巴细胞亚群的局部评估。

WC1 +γδT淋巴细胞亚群的局部表达已通过免疫组织化学方法在天然受鸟分枝杆菌亚种感染的母牛中不同类型的病变处进行了评估。副结核病(地图)和未感染的对照动物。被感染的牛处于副结核病的潜伏期/亚临床期(局灶性病变)或临床期(弥漫性脓杆菌和多杆菌形式)。为了评估细胞分布,在固有层,肠道相关淋巴样组织和粘膜下层进行了差异细胞计数。不论病变类型如何,在所有感染动物中均观察到WC1 +γδT细胞的数量显着增加。具有局灶性病变的母牛显示的标记细胞数量高于具有弥散性形式的母牛,在这两种类型之间没有发现差异。在固有层中可以看到感染动物中免疫标记淋巴细胞阳性数量的这种增加,在具有局灶性病变的动物中则更高。虽然在淋巴组织中没有观察到数量上的差异,但在具有局灶性病变的动物中,WC1 +γδT细胞倾向于位于肉芽肿的周围。这些发现表明WC1 +γδT淋巴细胞在牛副结核中具有促炎作用,这可能在遏制淋巴组织局灶性肉芽肿中Map感染的遏制中起着重要作用,有助于防止向形成这种疾病的弥散性形式发展。临床体征。虽然在淋巴组织中没有观察到数量上的差异,但在具有局灶性病变的动物中,WC1 +γδT细胞倾向于位于肉芽肿的周围。这些发现表明WC1 +γδT淋巴细胞在牛副结核中具有促炎作用,这可能在遏制淋巴组织局灶性肉芽肿中Map感染的遏制中起着重要作用,有助于防止向形成这种疾病的弥散性形式发展。临床体征。虽然在淋巴组织中没有观察到数量上的差异,但在具有局灶性病变的动物中,WC1 +γδT细胞倾向于位于肉芽肿的周围。这些发现表明WC1 +γδT淋巴细胞在牛副结核中具有促炎作用,这可能在遏制淋巴组织局灶性肉芽肿中Map感染的遏制中起着重要作用,有助于防止向形成这种疾病的弥散性形式发展。临床体征。
更新日期:2020-01-17
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