Cattle, bison and buffaloes are susceptible to Mycobacterium bovis, the causative agent for bovine tuberculosis. Accurate and timely identification of infected animals is critical for improved management and control of disease in these species. Bovids develop humoral immune responses to M. bovis infection making serological tests attractive for tuberculosis screening. However, optimization and validation of antibody assays designed for various animal species require understanding of antigen recognition patterns in each target host. The objective of this study was to characterize serological reactivity profiles generated by cattle, American bison, and African buffaloes in tuberculosis. Serum samples from M. bovis-infected animals were tested for the presence of IgM and IgG antibodies to MPB70/MPB83 and CFP10/ESAT6 chimeric proteins using Dual-Path Platform technology. All three host species showed IgG responses of higher magnitude and frequency than IgM responses; further, IgM seroreactivity was limited to MPB70/MPB83, whereas IgG antibodies recognized both test antigens. In cattle, the IgM and IgG responses were elicited mainly by MPB70/MPB83, whereas bison and buffaloes showed similar IgG seroreactivity rates for MPB70/MPB83 and CFP10/ESAT6 antigens. The findings demonstrate distinct patterns of predominant antigen recognition by different bovid species in M. bovis infection.

中文翻译：

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来自牛分枝杆菌感染的三个牛宿主的血清抗体的差异抗原识别。

牛，野牛和水牛容易感染牛结核分枝杆菌，牛结核分枝杆菌是牛分枝杆菌的病原体。准确及时地识别出感染动物对于改善这些物种的疾病管理和控制至关重要。牛科动物对牛分枝杆菌感染产生体液免疫反应，使血清学检查对结核病筛查具有吸引力。但是，为各种动物设计的抗体测定的优化和验证需要了解每个靶标宿主中的抗原识别模式。这项研究的目的是鉴定牛，美洲野牛和非洲水牛在结核病中产生的血清学反应性。M的血清样本 使用Dual-Path Platform技术测试了牛感染的动物是否存在针对MPB70 / MPB83和CFP10 / ESAT6嵌合蛋白的IgM和IgG抗体。所有三种宿主物种均显示出比IgM反应更高的强度和频率的IgG反应。此外，IgM的血清反应活性仅限于MPB70 / MPB83，而IgG抗体可识别两种测试抗原。在牛中，IgM和IgG应答主要由MPB70 / MPB83引起，而野牛和水牛对MPB70 / MPB83和CFP10 / ESAT6抗原显示相似的IgG血清反应率。这些发现证明了牛分枝杆菌感染中不同牛种对抗原的主要识别方式不同。而IgG抗体识别两种测试抗原。在牛中，IgM和IgG应答主要由MPB70 / MPB83引起，而野牛和水牛对MPB70 / MPB83和CFP10 / ESAT6抗原显示相似的IgG血清反应率。这些发现证明了牛分枝杆菌感染中不同牛种对抗原的主要识别方式不同。而IgG抗体识别两种测试抗原。在牛中，IgM和IgG应答主要由MPB70 / MPB83引起，而野牛和水牛对MPB70 / MPB83和CFP10 / ESAT6抗原显示相似的IgG血清反应率。这些发现证明了牛分枝杆菌感染中不同牛种对抗原的主要识别方式不同。