The treatment of Helicobacter pylori usually fails due to their ability to form biofilms and resistance to antibiotics. This might potentially lead to gastric carcinoma and mucosa‐associated lymphoid tissue lymphoma. In the present study, we elucidate the potential role of N‐acylhomoserine lactonase stabilized silver nanoparticles (AiiA‐AgNPs) in treating biofilms produced by H. pylori. AiiA‐AgNPs inhibited quorum sensing (QS) by degradation of QS molecules, thereby reducing biofilm formation, urease production, and altering cell surface hydrophobicity of H. pylori. AiiA‐AgNPs showed no cytotoxic effects on RAW 264.7 macrophages at the effective concentration (1–5 µM) of antibiofilm activity. In addition, AiiA‐AgNP in high concentration (80–100 µM) exhibited cytotoxicity against HCT‐15 carcinoma cells, depicting its therapeutic role in treating cancer.

中文翻译：

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N-酰基高丝氨酸内酯酶银纳米颗粒对幽门螺杆菌群体感应系统的影响：对抗生物膜形成的潜在策略

幽门螺杆菌的治疗通常会因它们形成生物膜的能力和对抗生素的耐药性而失败。这可能会导致胃癌和黏膜相关淋巴组织淋巴瘤。在本研究中，我们阐明了 N-酰基高丝氨酸内酯酶稳定的银纳米粒子（AiiA-AgNPs）在治疗幽门螺杆菌产生的生物膜中的潜在作用。AiiA-AgNPs 通过降解 QS 分子抑制群体感应 (QS)，从而减少生物膜形成、脲酶产生，并改变幽门螺杆菌的细胞表面疏水性。AiiA-AgNPs 在抗生物膜活性的有效浓度 (1–5 µM) 下对 RAW 264.7 巨噬细胞没有细胞毒性作用。此外，高浓度 (80–100 µM) 的 AiiA-AgNP 对 HCT-15 癌细胞表现出细胞毒性，