Some studies have highlighted benefits for Lobesia botrana by adding Botrytis cinerea mycelium to an artificial larval diet and have suggested a mutualistic relationship between the two organisms on grapevine, hypothesizing that fungal sterols were the nutritional factor involved. Because the nutritional quality of an artificial diet should be similar to grapes to allow extrapolation of the results to the field conditions, in the current study L. botrana larval performance was compared when larvae were fed on grapes (berries) or two artificial diets either with or without enrichment with B. cinerea. Based on sterol analysis, the two artificial diets had high cholesterol content, but relative to berries showed comparable and low phytosterol contents, respectively (high‐ and low‐phytosterol, HPh, and LPh). While larval fitness on the HPh diet was similar to berries, the LPh diet led to higher mortality and worse larval performance. The addition of the fungus compensated for the shortage in the LPh diet but did not improve the HPh diet. Supplementing the LPh diet with linoleic acid, which is supplied also from B. cinerea, partially improved larval performance. In a field experiment, females did not show any egg‐laying preferences towards naturally botrytized bunches. The positive effect of B. cinerea on the moth's next generation that is reported in the literature could be a consequence of fungus developed inside berry tunnels bored by larvae. Therefore, based on our data and previous reports the existence of a mutualistic relationship between L. botrana and B. cinerea is not well‐founded.

中文翻译：

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是否存在Boloba和灰葡萄孢之间的互惠关系？

一些研究通过在人工幼虫饮食中添加灰葡萄孢菌丝菌体，突出了对波贝娜的好处，并提出了葡萄中两种生物之间的相互关系，并假设真菌固醇是其中的营养因子。由于人工饮食的营养质量应与葡萄相似，以便将结果外推到田间条件，因此在本研究中，比较了用葡萄（浆果）或两种人工饮食饲喂幼虫时的博特兰乳杆菌幼虫性能。或不富集灰质芽孢杆菌。根据固醇分析，两种人工饮食的胆固醇含量较高，但相对于浆果而言，植物甾醇含量相近和较低（高植物甾醇和低植物甾醇，HPh和LPh）。虽然HPh饮食的幼虫适应性与浆果相似，但LPh饮食导致较高的死亡率和较差的幼体性能。真菌的添加弥补了LPh饮食的不足，但并未改善HPh饮食。向LPh饮食补充亚油酸（也可从灰质芽孢杆菌（B. cinerea）提供）可部分改善幼虫的性能。在田间试验中，雌性对自然晶化的串未表现出任何产卵的喜好。灰葡萄孢的积极作用文献中报道的蛾的下一代可能是由于幼虫在浆果隧道内生长的真菌造成的。因此，根据我们的数据和先前的报道，尚不存在建立博特拉乳酸杆菌和灰葡萄孢之间相互关系的依据。