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Molecular study and genotyping of Cryptosporidium baileyi and Cryptosporidium parvum from free-range and commercial broiler chickens in Guilan province, Iran.
Comparative Immunology, Microbiology and Infectious Diseases ( IF 2 ) Pub Date : 2020-01-10 , DOI: 10.1016/j.cimid.2019.101411
Parisa Shahbazi 1 , Armin Aligolzadeh 1 , Monireh Khordadmehr 1 , Hosein Hashemzadeh Farhang 2 , Farzad Katiraee 1
Affiliation  

Cryptosporidiosis acutely impacts the digestive and/or respiratory tract of the birds in many species of various orders. More importantly, it is also well known as a significant zoonotic disease, which can lead to diarrhea in humans and livestock. Regarding increasing demand for free-range products and increasing the number of free-range poultry farms, the present paper evaluated histopathological and molecular detection of Cryptosporidium baileyi and Cryptosporidium parvum in free-range and commercial broiler chickens in the north part of Iran. For this purpose, 100 fecal and tissue samples of the chickens in Guilan province were collected. After microscopic examination using Ziehl-Neelsen staining, molecular analyses of the fecal samples were processed by Nested-PCR targeting the 18S rRNA gene followed by sequencing of the amplicons and phylogenetic analyses. Eventually, the tissue samples were studied for histological lesions. Findings demonstrated the presence of Cryptosporidium baileyi and Cryptosporidium parvum in 6 % and 2 % of fecal samples, respectively. This is the first identification of C.parvum in avian hosts in Iran, and for the first time, C.baileyi and C.parvum are shown in native free-range chickens in Iran. All of the PCR positive birds with clinical symptoms showed gross lesions of respiratory infections. There was no significant difference between infection rate in free-range and commercial broiler chickens; however, the infection rate was significantly higher in chickens <25 days old. To conclude, we present here a notable Cryptosporidium infection rate in the free-range chicks in Iran, which notify the role of this host as a reservoir and should be more noted due to the economic and zoonotic importance.

中文翻译:

伊朗桂兰省自由放养和商业性肉鸡的百日隐孢子虫和小隐孢子虫的分子研究和基因分型。

隐孢子虫病会严重影响各种订单种类的鸟类的消化道和/或呼吸道。更重要的是,它也被称为重要的人畜共患病,可导致人类和牲畜腹泻。关于对散养产品的需求增加和散养家禽养殖场的数目增加,本论文评估了伊朗北部散养和商业肉鸡的百日隐孢子虫和小隐孢子虫的组织病理学和分子检测。为此,在桂兰省收集了100只鸡的粪便和组织样本。在使用Ziehl-Neelsen染色镜检后,粪便样品的分子分析通过以18S rRNA基因为靶标的Nested-PCR进行,然后对扩增子进行测序并进行系统发育分析。最终,研究了组织样品的组织学损伤。研究发现,分别在6%和2%的粪便样品中存在百日隐孢子虫和小孢隐孢子虫。这是伊朗鸟类宿主中首次鉴定出小隐孢子虫,并且首次在伊朗的本地散养鸡中显示了小隐孢子虫和小隐孢子虫。所有具有临床症状的PCR阳性禽类均显示出呼吸道感染的严重损害。散养和商业肉鸡的感染率没有显着差异。但是,<25天大的鸡的感染率明显更高。最后,
更新日期:2020-01-10
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