Monitoring of acute phase proteins such as serum amyloid A at gene expression level may provide quick information about immune status of the host and its susceptibility towards common infections. Present study was carried out to evaluate and compare the mRNA expression of SAA gene in Rhode Island Red chicken (RIR) and Japanese quails using real time PCR analysis in response to inactivated Salmonella gallinarum culture. The results showed that expression of SAA gene was approximately 17-33 folds higher in case of birds administered with bacterial culture when compared to un-inoculated controls and expression was higher and quicker in case of quails than RIR chicken. The SAA genes from chicken and quail were cloned and upon sequence analysis it was observed that deduced amino acid sequence of SAA from chicken and quails were having approximately seven percent variation which might have significance in function of this protein in these species.

中文翻译：

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鸡和鹌鹑血清淀粉样蛋白A（SAA）mRNA表达对细菌压力的反应。

在基因表达水平上监测急性期蛋白（例如血清淀粉样蛋白A）可以提供有关宿主免疫状态及其对常见感染的敏感性的快速信息。本研究旨在通过实时PCR分析来评估和比较罗德岛红鸡（RIR）和日本鹌鹑中SAA基因的mRNA表达，以响应灭活鸡沙门氏菌培养。结果表明，与未接种的对照相比，在进行细菌培养的鸟类中，SAA基因的表达高出约17-33倍，而在鹌鹑中，SAA基因的表达则比RIR鸡更高，更快。