Objective. To evaluate the possible biological effect of allogenic mesenchymal stem cells (MSCs) combined with tissue fusion technology on the anastomosis. Methods. Sixteen pigs were divided into a 7 d group and 14 d group, each of which was further subdivided into an MSC-treated group and a control group. Five anastomoses per animal were established using LigaSure ForceTriad (Covidien, MA, USA), a tissue sealing system. Cell migration and tissue-specific differentiation potency, in addition to potential cytokine and genetic changes, were investigated. Results. There were no significant between-group differences in postoperative complications and anastomosis burst pressure. The number of proliferating cell nuclear antigen- (PCNA-) positive cells was significantly higher in the MSC-treated group as compared with that in the control group (). Labeled MSCs were found in the mucosal layer, villus, and lamina propria, as well as in the lamina muscularis mucosae, where they exhibited characteristics of smooth muscle cells. Conclusions. Grafted MSCs significantly promoted epithelial and connective cell proliferation and maintained their cell migration capacity and differentiation potential in the fused anastomotic tissues, without causing severe postoperative complications.

中文翻译：

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间充质干细胞联合组织融合技术促进猪肠吻合术创面愈合。

客观。评价同种异体间充质干细胞（MSCs）联合组织融合技术对吻合口可能产生的生物学效应。方法。16头猪分为7 d组和14 d组，每组进一步细分为MSC处理组和对照组。使用组织密封系统 LigaSure ForceTriad（Covidien，MA，USA）为每只动物建立五个吻合口。除了潜在的细胞因子和遗传变化外，还研究了细胞迁移和组织特异性分化能力。结果. 术后并发症和吻合口爆裂压力无显着组间差异。与对照组相比，MSC治疗组的增殖细胞核抗原（PCNA-）阳性细胞数显着增加（）。在黏膜层、绒毛和固有层以及黏膜肌层中发现了标记的 MSC，在那里它们表现出平滑肌细胞的特征。结论。移植的间充质干细胞显着促进上皮细胞和结缔细胞增殖，并保持其在融合吻合组织中的细胞迁移能力和分化潜能，而不会引起严重的术后并发症。