Background. Adipose tissue-derived stromal cells (ADSCs) have great potential for cell-based therapies, including tissue engineering. However, various factors can influence the characteristics of isolated ADSCs. Methods. We studied the influence of the harvesting site, i.e., inner thigh (), outer thigh (), and abdomen (), and of negative pressure, i.e., low (-200 mmHg) and high (-700 mmHg), on the characteristics of isolated ADSCs. We counted initial yields of attached cells after isolation. In subsequent passage, we studied the number, viability, diameter, doubling time, mitochondrial activity, and CD surface markers of isolated ADSCs. Results. We revealed higher initial cell yields from the outer thigh region than from the abdomen region. Negative pressure did not influence the cell yields from the outer thigh region, whereas the yields from the abdomen region were higher under high negative pressure than under low negative pressure. In the subsequent passage, in general, no significant relationship was identified between the different negative pressure and ADSC characteristics. No significant difference was observed in the characteristics of thigh ADSCs and abdomen ADSCs. Only on day 1, the diameter was significantly bigger in outer thigh ADSCs than in abdomen ADSCs. Moreover, we noted a tendency of thigh ADSCs (i.e., inner thigh+outer thigh) to reach a higher cell number on day 7. Discussion. The harvesting site and negative pressure can potentially influence initial cell yields from lipoaspirates. However, for subsequent in vitro culturing and for use in tissue engineering, it seems that the harvesting site and the level of negative pressure do not have a crucial or limiting effect on basic ADSC characteristics.

中文翻译：

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负压和收获部位对来自脂肪抽吸物的人类脂肪组织来源的基质细胞特征的影响。

背景。脂肪组织来源的基质细胞 (ADSC) 在包括组织工程在内的基于细胞的治疗方面具有巨大潜力。然而，各种因素会影响离体 ADSCs 的特性。方法。我们研究了采集部位的影响，即大腿内侧（),大腿外侧 ()和腹部 ()和负压，即低 (-200 mmHg) 和高 (-700 mmHg)，对离体 ADSC 的特性。我们计算了分离后附着细胞的初始产量。在随后的段落中，我们研究了分离的 ADSC 的数量、活力、直径、倍增时间、线粒体活性和 CD 表面标志物。结果. 我们发现大腿外侧区域的初始细胞产量高于腹部区域。负压不影响大腿外侧区域的细胞产量，而腹部区域的产量在高负压下高于低负压下。在随后的段落中，一般来说，没有发现不同的负压和 ADSC 特性之间有显着的关系。大腿ADSCs和腹部ADSCs的特征无显着差异。仅在第 1 天，大腿外侧 ADSC 的直径明显大于腹部 ADSC。此外，我们注意到大腿 ADSC（即大腿内侧 + 大腿外侧）在第 7 天达到更高细胞数的趋势。讨论. 收获部位和负压可能会影响脂肪抽吸物的初始细胞产量。然而，对于随后的体外培养和用于组织工程，似乎收获部位和负压水平对基本的 ADSC 特征没有关键或限制性影响。