Regulatory T cells (T-reg) are important components of immune system required to understand the mechanistic details of cancer immunity and autoimmune diseases. However, reliable and efficient methods of regulatory T cell expansion have not been established yet. Here, we show that a human peripheral blood mononuclear cell (PBMNC) derived blood-born hematosphere (BBHS) culture without cytokine treatment increased T-reg and T helper 2 cell (Th2) populations in the cell suspension. We found that the isolated cells suspended around the hematospheres expressed T helper cell markers. Importantly, the Foxp3+/CD25+ T-reg and IL-4+/CD25+ Th2 cell populations in the cell suspension were greatly expanded during hematosphere culture. Through ELISA analysis of the supernatant, we showed that secretion of Th2-related cytokines such as IL-5, IL-10, and IL-13 also increased. Taken together, hematosphere culture is a good method for ex vivo expansion of T-reg and Th2, which can provide therapeutic benefits for the treatment of immune disorders.

中文翻译：

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使用血球培养物对调节性 T 细胞和 T 辅助 2 细胞进行体外扩增。

调节性 T 细胞 (T-reg) 是了解癌症免疫和自身免疫疾病的机制细节所需的免疫系统的重要组成部分。然而，尚未建立可靠和有效的调节性 T 细胞扩增方法。在这里，我们展示了未经细胞因子处理的人外周血单核细胞 (PBMNC) 衍生的血源性血球 (BBHS) 培养物增加了细胞悬浮液中的 T-reg 和 T 辅助 2 细胞 (Th2) 种群。我们发现悬浮在血球周围的分离细胞表达了 T 辅助细胞标志物。重要的是，在血球培养过程中，细胞悬液中的 Foxp3+/CD25+ T-reg 和 IL-4+/CD25+ Th2 细胞群大大增加。通过对上清液的 ELISA 分析，我们发现 Th2 相关细胞因子如 IL-5、IL-10、IL-13 也增加。综上所述，血球培养是 T-reg 和 Th2 体外扩增的好方法，可为免疫疾病的治疗提供治疗益处。