Previously, it was found that the hepatitis C virus NS5A interacted with ACBD3 in a genotype‐dependent manner. However, the region in NS5A responsible for association with ACBD3 is not clear. Domain I of NS5A was identified as critical for ACBD3 binding. By comparing the differences of amino acids in domain I from different genotypes of NS5A, it was found that key amino acids potentially corresponded to the affinity of the NS5A‐ACBD3 interaction. The findings not only revealed that domain I of NS5A associates with ACBD3 but they also shed mechanistic light on how NS5A is associated with ACBD3 in a genotype‐dependent manner.

中文翻译：

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丙型肝炎病毒 NS5A 的域 I 以基因型依赖性方式与 ACBD3 相关。

此前，人们发现丙型肝炎病毒 NS5A 与 ACBD3 以基因型依赖性方式相互作用。然而，NS5A 中负责与 ACBD3 关联的区域尚不清楚。NS5A 的域 I 被确定为对 ACBD3 结合至关重要。通过比较不同基因型 NS5A 的结构域 I 氨基酸的差异，发现关键氨基酸可能对应于 NS5A-ACBD3 相互作用的亲和力。研究结果不仅揭示了 NS5A 的结构域 I 与 ACBD3 相关，而且还揭示了 NS5A 如何以基因型依赖性方式与 ACBD3 相关的机制。