This paper describes the continuation of studies that demonstrated the suitability of CP-Tes solution as a medium for the introduction and removal of dimethyl sulfoxide in rabbit common carotid arteries and established the kinetics of cryoprotectant permeation in that tissue. In this paper we report the tolerance of rabbit common carotid artery to dimethyl sulfoxide, in concentrations up to 30% (w/w), using a technique of exposure that was designed to control osmotic stress. The maximum concentration achieved without damage was 15% (w/w). Vessels were then equilibrated with 15% dimethyl sulfoxide and cooled to -80°C at 0.22, 0.69, 2.15, or 9.63°C/min: they were then transferred to the gas phase of a liquid nitrogen refrigerator {temperature below -160°C) for storage. Thawing was carried out in a 37°C water bath. The optimum rate of cooling for these conditions was found to be 0.69°C/min. The maximal recovery of contractile force in response to 10-6 M norepinephrine was 30-40%; relaxation to acetylcholine (an endothelium-mediated function) was 80% of control, and an estimated 71% of endothelial cells survived with minimal ultrastructural change.

中文翻译：

---

兔颈总动脉的冷冻保存：二甲基亚砜浓度和冷却速率的优化

本文描述了研究的延续，这些研究证明了 CP-Tes 溶液作为介质在兔颈总动脉中引入和去除二甲基亚砜的适用性，并建立了该组织中冷冻保护剂渗透的动力学。在本文中，我们报告了兔颈总动脉对二甲基亚砜的耐受性，浓度高达 30% (w/w)，使用旨在控制渗透压力的暴露技术。在没有损坏的情况下达到的最大浓度为 15% (w/w)。然后将容器用 15% 二甲亚砜平衡并以 0.22、0.69、2.15 或 9.63°C/min 的速度冷却至 -80°C：然后将它们转移到液氮冰箱的气相中（温度低于 -160°C ) 用于存储。解冻在 37°C 水浴中进行。发现这些条件的最佳冷却速率为 0.69°C/min。响应 10-6 M 去甲肾上腺素的收缩力的最大恢复为 30-40%；乙酰胆碱松弛（内皮介导的功能）是对照的 80%，估计有 71% 的内皮细胞存活，超微结构变化最小。