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Trichostatin A alleviated ovarian tissue damage caused by cigarette smoke exposure.
Reproductive Toxicology ( IF 3.3 ) Pub Date : 2020-01-24 , DOI: 10.1016/j.reprotox.2020.01.006
Fang Li 1 , Jingjing Ding 1 , Yanfei Cong 1 , Bo Liu 1 , Jianing Miao 1 , Di Wu 2 , Lili Wang 1
Affiliation  

Cigarette smoke (CS) has a negative impact on women's health and fertility. Studies have shown that histone deacetylases 1 and 2 (HDAC1/2) were involved in oocyte development. However, the roles of HDAC1/2 in ovarian toxicity caused by CS exposure and the therapeutic potential of trichostatin A (TSA, a HDAC inhibitor) for ovarian tissue damage have not been investigated. In this study, Female C57BL/6 mice were exposed to CS from six cigarettes mixed with indoor air for 120 min (one cigarette for 20 min) using a whole-body mainstream smoke exposure system twice daily for 30 days. TSA (0.6 mg/kg body weight) was injected intraperitoneally into mice in the Control + TSA group and CS + TSA group every two days for 30 days. We found that exposure to CS resulted in ovarian tissue damage and HDAC1/2 over-expression. TSA alleviated the structural changes of ovarian tissue induced by smoking and prevented the activation of HDAC1/2. Exposure to CS caused autophagy inhibition and pyroptosis activation. TSA treatment restored the expression of autophagy-associated proteins and decreased the levels of pyroptosis-related proteins induced by CS exposure. The TSA effect may be mediated by inhibition of HDAC1/2 involved in autophagy and pyroptosis process.

中文翻译:

曲古他汀A减轻了香烟烟雾引起的卵巢组织损伤。

香烟烟雾(CS)对妇女的健康和生育能力有负面影响。研究表明,组蛋白脱乙酰基酶1和2(HDAC1 / 2)与卵母细胞发育有关。但是,尚未研究过HDAC1 / 2在CS暴露引起的卵巢毒性中的作用以及曲古抑菌素A(TSA,HDAC抑制剂)对卵巢组织损伤的治疗潜力。在这项研究中,雌性C57BL / 6小鼠使用全身主流烟雾暴露系统从六支与室内空气混合的香烟中暴露于CS中120分钟(一支香烟20分钟),每天30次。在30天内,每两天将TSA(0.6mg / kg体重)腹膜内注射到对照组+ TSA组和CS + TSA组的小鼠中。我们发现接触CS会导致卵巢组织损伤和HDAC1 / 2过表达。TSA减轻了吸烟引起的卵巢组织的结构变化,并阻止了HDAC1 / 2的激活。接触CS会引起自噬抑制和焦磷酸激活。TSA处理恢复了自噬相关蛋白的表达,并降低了CS暴露诱导的发烧相关蛋白的水平。TSA效应可能通过抑制自噬和发烧过程中涉及的HDAC1 / 2介导。
更新日期:2020-03-30
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