The cre-loxP system has been revolutionary in the field of immunology. The technology enables genetic deletion in mice with unprecedented precision. It is therefore now widely used to investigate gene functions in animal models of disease, and in fundamental studies of the immune response. This widespread adoption of cre-loxP technology has allowed a thorough investigation of its strengths and weaknesses. Here we highlight an important paper which not only describes potential problems with the commonly-used screening procedures used when identifying offspring of the correct genotype, but also describes how this screening can be improved to ensure that the right animals are produced.

中文翻译：

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我们应该更信服吗？重组的警示故事。

cre-loxP 系统在免疫学领域是革命性的。该技术能够以前所未有的精确度对小鼠进行基因删除。因此，它现在被广泛用于研究疾病动物模型中的基因功能，以及免疫反应的基础研究。cre-loxP 技术的广泛采用使得对其优势和劣势的彻底调查成为可能。在这里，我们重点介绍了一篇重要论文，该论文不仅描述了识别正确基因型后代时使用的常用筛选程序的潜在问题，还描述了如何改进这种筛选以确保生产出正确的动物。