The study of intrinsic phosphorylation dynamics and kinetics in the context of complex protein architecture in vivo has been challenging: Method limitations have prevented significant advances in the understanding of the highly variable turnover of phosphate groups, synergy, and cooperativity between P-sites. However, over the last decade, powerful analytical technologies have been developed to determine the full catalog of the phosphoproteome for many species. The curated databases of phospho sites found by mass spectrometry analysis and the computationally predicted sites based on the linear sequence of kinase motifs are valuable tools. They allow investigation of the complexity of phosphorylation in vivo, albeit with strong discrepancies between different methods. A series of hypothetical scenarios on combinatorial processive phosphorylation is proposed that are likely unverifiable with current methodologies. These proposed a priori postulates could be considered as possible extensions of the known schemes of the activation/inhibition signaling process in vivo.

中文翻译：

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蛋白质磷酸化动力学：由于当前方法学的局限性，尚未被探索：进行性磷酸化动力学。

在体内复杂蛋白质结构的背景下固有的磷酸化动力学和动力学的研究具有挑战性：方法的局限性阻止了对磷酸基团，协同作用和P位之间的协同性的高度可变的理解的重大进步。然而，在过去的十年中，已经开发了强大的分析技术来确定许多物种的磷酸化蛋白质组的完整目录。通过质谱分析发现的磷酸位点的精选数据库和基于激酶基序线性序列的计算预测位点是有价值的工具。它们允许研究体内磷酸化的复杂性，尽管不同方法之间存在很大差异。提出了一系列关于组合进行性磷酸化的假想场景，这些场景可能无法用当前的方法论进行验证。这些提出的先验假设可以被认为是体内激活/抑制信号传导过程的已知方案的可能扩展。