The first genomic study of Mediterranei clade using five type strains (V. mediterranei, V. maritimus, V. variabilis, V. thalassae, and V. barjaei) and fourteen reference strains isolated from marine organisms, seawater, water and sediments of the sea was performed. These bacterial strains were characterised by means of a polyphasic approach comprising 16S rRNA gene, multilocus sequence analysis (MLSA) of 139 single-copy genes, the DNA G + C content, ANI, and in silico phenotypic characterisation. We found that the species of the Mediterranei clade formed two separate clusters based in 16S rRNA gene sequence similarity, MLSA, OrthoANI, and Codon and Amino Acid usage. The Mediterranei clade species showed values between 76 and 95% for ANIb, 84 and 95% for ANIm. The core genome consisted of 2057 gene families and the pan-genome of 13,094 gene families. Based on the genomic analyses performed, the Mediterranei clade can be divided in two clusters, one with the strains of V. maritimus, V. variabilis and two potential new species, and the other cluster with the strains of V. mediterranei, V. thalassae, and V. barjaei.

中文翻译：

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弧菌属地中海细菌进化枝的基因组分类法（丙种杆菌）。

使用五种类型的菌株（V. mediterranei，V。maritimus，V。variabilis，V。thalassae和V. barjaei）和从海洋生物，海水，水和海洋沉积物中分离的14种参考菌株对地中海枝条进行首次基因组研究被执行了。这些细菌菌株通过多相方法进行表征，该方法包括16S rRNA基因，139个单拷贝基因的多基因座序列分析（MLSA），DNA G + C含量，ANI和计算机模拟表型表征。我们发现，根据16S rRNA基因序列相似性，MLSA，OrthoANI以及密码子和氨基酸使用情况，地中海进化枝的物种形成了两个单独的簇。地中海枝条的ANIb值介于76％至95％之间，ANIm的值介于84％至95％之间。核心基因组由2057个基因家族和13个全基因组组成 094个基因家族。根据进行的基因组分析，可以将地中海枝条分为两个簇，一个簇具有海弧菌，变异弧菌和两个潜在的新物种，另一个簇与水生弧菌，拟南芥的菌株和V. barjaei。