A Gram-negative and facultative anaerobic bacterium, designated strain SN4T, was isolated from the stool sample of an obese Amazonian patient. The new isolate was characterized by the taxonogenomics approach. The strain SN4T was beige-colored, circular and not haemolytic. Cells are rod shaped and motile with several flagella. Strain SN4T grows optimally at pH 7 and can survive in the presence of a saline concentration of up to 75 g/l NaCl. The 16S ribosomal RNA gene sequence analysis of the novel strain SN4T showed 95.28% similarity in nucleotide sequence with Gorillibacterium massiliense G5T, the phylogenetically closest neighbor and the type species of this genus. Anteiso-C15:0, iso-C15:0 and C16:0 were found as the major components in the cellular fatty acid analysis of this isolate. The genomic draft of strain SN4T is 5,263,742 bp long with 53.33% of G+C content. The differences in physiological, biochemical characteristics and phylogenetic and genomic data make it possible to clearly distinguish the strain SN4T from G. massiliense G5T. Based on the taxonogenomic description and the phenotypic and biochemical characteristics of this bacterium presented in this article, we propose the SN4T strain (= CSUR P2011 = DSM 100,698) as a new species, Gorillibacterium timonense sp. nov.

中文翻译：

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Gorillibacterium timonense sp. 11 月，与肥胖患者隔离

从一名肥胖亚马逊患者的粪便样本中分离出一种革兰氏阴性兼性厌氧菌，命名为 SN4T 菌株。新分离株的特点是分类基因组学方法。SN4T 菌株呈米色、圆形且不溶血。细胞呈棒状，有数根鞭毛，可活动。菌株 SN4T 在 pH 7 下最佳生长，并且可以在浓度高达 75 g/l NaCl 的盐水中存活。新菌株SN4T的16S核糖体RNA基因序列分析表明，与该属的系统发育最近邻和模式种Gorillibacterium massiliense G5T的核苷酸序列相似度为95.28%。Anteiso-C15:0、iso-C15:0 和 C16:0 被发现是该分离株细胞脂肪酸分析中的主要成分。菌株 SN4T 的基因组草图长 5,263,742 bp，其中 53 个。G+C 含量的 33%。生理、生化特征以及系统发育和基因组数据的差异使得可以清楚地区分菌株 SN4T 和 G. massiliense G5T。基于本文中介绍的这种细菌的分类基因组学描述以及表型和生化特征，我们提出 SN4T 菌株（= CSUR P2011 = DSM 100,698）作为一个新物种，Gorillibacterium timonense sp。十一月 Gorillibacterium timonense sp. 十一月 Gorillibacterium timonense sp. 十一月