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Expression of gynoecium patterning transcription factors in Aristolochia fimbriata (Aristolochiaceae) and their contribution to gynostemium development.
EvoDevo ( IF 4.1 ) Pub Date : 2020-02-17 , DOI: 10.1186/s13227-020-00149-8
Pablo Peréz-Mesa 1 , Clara Inés Ortíz-Ramírez 2 , Favio González 3 , Cristina Ferrándiz 2 , Natalia Pabón-Mora 1
Affiliation  

Background In Aristolochia (Aristolochiaceae) flowers, the congenital fusion of the anthers and the commissural, stigmatic lobes forms a gynostemium. Although the molecular bases associated to the apical-basal gynoecium patterning have been described in eudicots, comparative expression studies of the style and stigma regulatory genes have never been performed in early divergent angiosperms possessing a gynostemium. Results In this study, we assess the expression of five genes typically involved in gynoecium development in Aristolochia fimbriata. We found that all five genes (AfimCRC, AfimSPT, AfimNGA, AfimHEC1 and AfimHEC3) are expressed in the ovary, the placenta, the ovules and the transmitting tract. In addition, only AfimHEC3, AfimNGA and AfimSPT are temporarily expressed during the initiation of the stigma, while none of the genes studied is maintained during the elaboration of the stigmatic surfaces in the gynostemium. Conclusions Expression patterns suggest that CRC, HEC, NGA and SPT homologs establish ovary and style identity in Aristolochia fimbriata. Only NGA,HEC3 and SPT genes may play a role in the early differentiation of the stigmatic lobes, but none of the genes studied seems to control late stigma differentiation in the gynostemium. The data gathered so far raises the possibility that such transient expression early on provides sufficient signal for late stigma differentiation or that unidentified late identity genes are controlling stigma development in the gynostemium. Our data does not rule out the possibility that stigmas could correspond to staminal filaments with convergent pollen-receptive surfaces.

中文翻译:

马兜铃 (Aristolochiaceae) 中雌蕊模式转录因子的表达及其对雌蕊发育的贡献。

背景 在马兜铃 (Aristolochiaceae) 花中,花药和连合柱头裂片的先天性融合形成合股掌。尽管在真双子叶植物中已经描述了与顶端 - 基底 gynoecium 图案相关的分子基础,但从未在具有合股草的早期发散被子植物中进行过样式和柱头调节基因的比较表达研究。结果在这项研究中,我们评估了通常参与马兜铃雌蕊发育的五个基因的表达。我们发现所有五个基因(AfimCRC、AfimSPT、AfimNGA、AfimHEC1 和 AfimHEC3)都在卵巢、胎盘、胚珠和传递道中表达。此外,只有 AfimHEC3、AfimNGA 和 AfimSPT 在柱头开始期间暂时表达,而在绞股蓝柱头表面的加工过程中,所研究的基因都没有被保留。结论 表达模式表明 CRC、HEC、NGA 和 SPT 同源物在马兜铃中建立了卵巢和花型特征。只有 NGA、HEC3 和 SPT 基因可能在柱头裂片的早期分化中起作用,但所研究的基因似乎都没有控制合股柱柱头分化的晚期。迄今为止收集的数据提出了这样一种可能性,即这种早期的瞬时表达为晚期柱头分化提供了足够的信号,或者未鉴定的晚期身份基因正在控制合股树中的柱头发育。我们的数据不排除柱头可能对应于具有收敛花粉接受表面的雄蕊花丝的可能性。
更新日期:2020-04-22
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