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Circular RNAs hsa_circ_0000479 in peripheral blood mononuclear cells as novel biomarkers for systemic lupus erythematosus.
Autoimmunity ( IF 3.5 ) Pub Date : 2020-02-24 , DOI: 10.1080/08916934.2020.1728529
Qing Luo 1 , Lu Zhang 2 , Le Fang 3 , Biqi Fu 4 , Yang Guo 1 , Zikun Huang 1 , Junming Li 1
Affiliation  

Circular RNAs (circRNAs) are a class of non-coding RNAs that play a crucial role in diagnosis and prognosis of systemic lupus erythematosus (SLE). However, circRNAs expression profiling in SLE from different reports are different. In this study, 11 circRNAs (hsa_circ_0000479, hsa_circ_0002316, hsa_circ_0000317, hsa_circ_0082688, hsa_circ_0082689, hsa_circ_0087798, hsa_circ_0008529, hsa_circ_0000787, hsa_circ_0021727, hsa_circ_0000175, and hsa_circ_0003694) which were found to be significantly up-regulated in both peripheral blood mononuclear cells (PBMCs) from SLE patients in our previous study, and T cells from SLE patients in previous literature, were chosen for validation by quantitative reverse transcription-polymerase chain reaction in PBMCs from 50 new-onset SLE patients, 24 new-onset rheumatoid arthritis (RA) patients, 24 new-onset ankylosing spondylitis (AS) patients, and 45 age- and sex-matched healthy controls (HC). The results validated that PBMCs hsa_circ_0000479, hsa_circ_0082688, and hsa_circ_0082689 were increased, while hsa_circ_0000175 was significantly decreased in SLE patients than that in RA patients, AS patients, and HC. The correlation analysis of these confirmed differentially expressed circRNAs showed that hsa_circ_0000479 was associated with C3 level and treatment, hsa_circ_0082688 was associated with anti-dsDNA level, hsa_circ_0082689 was associated with anti-dsDNA level, anti-nuclesome frequency and treatment. Receiver operating characteristic curve anaylsis suggested that hsa_circ_0000479 has significant value in distinguishing SLE from AS patients, RA patients, and HC (AUC = 0.825, p < .001). Moreover, the hsa_circ_0000479-anti-dsDNA combination model could effectively discriminate the SLE group and the control groups (RA + AS + HC), with a sensitivity of 86.00% (43/50), a specificity of 100.00% (93/93), and an accuracy of 95.10% (136/143). This study suggested that hsa_circ_0000479 in PBMC and hsa_circ_0000479-anti-dsDNA combination model may serve as potential biomarkers for SLE diagnosis and evaluation of therapeutic effect.

中文翻译:

外周血单核细胞中的环状RNA hsa_circ_0000479作为系统性红斑狼疮的新型生物标记。

环状RNA(circRNA)是一类非编码RNA,在系统性红斑狼疮(SLE)的诊断和预后中起着至关重要的作用。但是,来自不同报道的SLE中circRNAs表达谱不同。在这项研究中,发现了11个circRNA(hsa_circ_0000479,hsa_circ_0002316,hsa_circ_0000317,hsa_circ_0082688,hsa_circ_0082689,hsa_circ_0087798,hsa_circ_0008529,hsa_circ_0000787,hsa_circ_0021727,hsa_circ_0000175和hsa_circ_00000175以及从周围向上显着分布的hsa_circ_00000和hsa_circ_00000都在外周血中我们通过筛选50例新发SLE患者,24例新发类风湿性关节炎(RA)患者的PBMC中的定量逆转录聚合酶链反应,选择了先前研究中的SLE患者和SLE患者中的T细胞进行验证。24例新发强直性脊柱炎(AS)患者,以及45例年龄和性别相匹配的健康对照(HC)。结果证实,与RA患者,AS患者和HC患者相比,SLE患者的PBMC hsa_circ_0000479,hsa_circ_0082688和hsa_circ_0082689升高,而hsa_circ_0000175显着降低。这些证实的差异表达的circRNA的相关性分析表明,hsa_circ_0000479与C3水平和治疗相关,hsa_circ_0082688与抗dsDNA水平相关,hsa_circ_0082689与抗dsDNA水平,抗核体频率和治疗相关。接收器工作特征曲线分析表明,hsa_circ_0000479在区分SLE与AS患者,RA患者和HC中具有重要价值(AUC = 0.825,p <.001)。此外,hsa_circ_0000479-anti-dsDNA组合模型可以有效区分SLE组和对照组(RA + AS + HC),敏感性为86.00%(43/50),特异性为100.00%(93/93),并且准确度为95.10%(136/143)。这项研究表明,PBMC中的hsa_circ_0000479和hsa_circ_0000479-anti-dsDNA组合模型可作为SLE诊断和评估疗效的潜在生物标志物。
更新日期:2020-02-24
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