Background. The aim of this study was to explore the role of hesperadin in intracerebral hemorrhage (ICH) mice, with the involvement of the mammalian ste20-like kinase 4 (MST4)/AKT signaling pathway. Methods. All mice were divided into four groups: sham group, sham+hesperidin group, ICH group, and ICH+hesperadin group. The effects of hesperadin were assessed on the basis of brain edema and neurobehavioral function. Furthermore, we observed MST4, AKT, phosphorylation of AKT (pAKT), and microtubule-associated protein light chain 3 (LC3) by western blotting. Protein localization of MST4 and LC3 was determined by immunofluorescence. Results. The expression of MST4 was upregulated at 12 h and 24 h after ICH. Brain edema was significantly decreased and neurological function was improved in the hesperadin treatment group compared to the ICH group (). Hesperadin decreases the expressions of MST and increases pAKT after ICH. Autophagy significantly increased in the ICH group, while hesperadin reduced this increase. Conclusion. Hesperadin provides neuroprotection against ICH by inhibiting the MST4/AKT signaling pathway.

中文翻译：

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MST4 激酶抑制剂橙皮素通过 MST4/AKT 通路减轻脑出血小鼠的自噬和行为障碍。

背景。本研究的目的是探讨橙皮素在脑出血 (ICH) 小鼠中的作用，其中涉及哺乳动物 ste20 样激酶 4 (MST4)/AKT 信号通路。方法。将所有小鼠分为4组：sham组、sham+橙皮苷组、ICH组、ICH+橙皮苷组。根据脑水肿和神经行为功能评估橙皮素的作用。此外，我们通过蛋白质印迹观察了 MST4、AKT、AKT (pAKT) 和微管相关蛋白轻链 3 (LC3) 的磷酸化。通过免疫荧光测定 MST4 和 LC3 的蛋白质定位。结果. 在 ICH 后 12 小时和 24 小时，MST4 的表达上调。与 ICH 组相比，橙皮苷治疗组脑水肿明显减轻，神经功能改善。）。橙皮素降低 MST 的表达并增加 ICH 后的 pAKT。ICH 组的自噬显着增加，而橙皮素减少了这种增加。结论。Hesperadin 通过抑制 MST4/AKT 信号通路提供针对 ICH 的神经保护作用。