BACKGROUND Children with congenital CMV infection (cCMV) shed virus in urine and saliva for prolonged periods of time. Outcome of cCMV varies from asymptomatic infection with no sequelae in most cases, to severe longterm morbidity. The factors associated with asymptomatic cCMV are not well defined. We evaluated the viral shedding in a cohort of infants with cCMV identified on newborn screening. In addition, we describe the distribution of viral genotypes in our cohort of asymptomatic infants and previous cohorts of cCMV children in the literature. METHODS Study population consisted of 40 children with cCMV identified in screening of 19,868 infants, a prevalence of 2/1000. The viral shedding was evaluated at 3 and 18 months of age by real-time CMV-PCR of saliva and plasma, and CMV culture of urine. CMV positive saliva samples were analyzed for genotypes for CMV envelope glycoproteins gB (UL55), and gH (UL75) by genotype specific real-time PCR, and gN (UL73) by cloning and sequencing RESULTS: At 3 months age 40/40 saliva and urine samples, and 19/40 plasma samples were positive for CMV. At 18 months age all urine samples tested (33/33), 9/37 of saliva samples, and 2/34 plasma samples were positive for CMV. The genotype distribution did not differ from the published data CONCLUSIONS: The urinary virus shedding is more persistent than salivary shedding in children with cCMV. The genotype distribution was similar to previous literature and does not explain the low disease burden of cCMV in our population.

中文翻译：

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先天性巨细胞病毒感染的病毒脱落和巨细胞病毒糖蛋白H（UL75），糖蛋白B（UL55）和糖蛋白N（UL73）基因型的分布。

背景技术患有先天性巨细胞病毒感染（cCMV）的儿童会长时间排尿和唾液中的病毒。从大多数情况下无后遗症的无症状感染到严重的长期发病，cCMV的结果有所不同。与无症状cCMV相关的因素尚不明确。我们评估了在新生儿筛查中发现的cCMV婴儿队列中的病毒脱落。此外，我们在文献中描述了无症状婴儿和先前cCMV儿童队列中病毒基因型的分布。方法研究人群由40名患cCMV的儿童组成，筛查了19,868名婴儿，患病率为2/1000。通过唾液和血浆的实时CMV-PCR以及尿液的CMV培养，对3个月和18个月大时的病毒脱落进行了评估。通过基因型特异性实时PCR分析CMV阳性唾液样品的CMV包膜糖蛋白gB（UL55）和gH（UL75）的基因型，并通过克隆和测序分析gN（UL73）的结果。尿液样本和19/40血浆样本CMV阳性。在18个月大时，所有测试的尿液样本（33/33），唾液样本的9/37和血浆样本的2/34均为CMV阳性。结论：在cCMV患儿中，泌尿病毒的脱落比唾液脱落更持久。基因型分布与以前的文献相似，不能解释我们人群中cCMV的低疾病负担。结果：在3个月大时40/40的唾液和尿液样本和19/40的血浆样本CMV阳性。在18个月大时，所有测试的尿液样本（33/33），唾液样本的9/37和血浆样本的2/34均为CMV阳性。结论：在cCMV患儿中，泌尿病毒的脱落比唾液脱落更持久。基因型分布与以前的文献相似，不能解释我们人群中cCMV的低疾病负担。结果：在3个月大时，唾液和尿液样本为40/40，血浆样本为19/40，CMV呈阳性。在18个月大时，所有测试的尿液样本（33/33），唾液样本的9/37和血浆样本的2/34均为CMV阳性。结论：在患有cCMV的儿童中，泌尿病毒的脱落比唾液脱落更持久。基因型分布与以前的文献相似，不能解释我们人群中cCMV的低疾病负担。结论：在患有cCMV的儿童中，泌尿病毒的脱落比唾液脱落更持久。基因型分布与以前的文献相似，不能解释我们人群中cCMV的低疾病负担。结论：cCMV患儿的尿液脱落比唾液脱落更持久。基因型分布与以前的文献相似，不能解释我们人群中cCMV的低疾病负担。