Objective: The posterior vagus nerve trunk innervates the entire small intestine, and elucidating its modulatory role in the IgA response was the aim of this study. Methods: Two groups of six male BALB/c mice underwent sham or posterior subdiaphragmatic vagotomy and were euthanized on the 14th postoperative day; then, the small intestines were dissected. The intestinal fluid was harvested for antibody analysis by ELISA, and cell suspensions from Peyer’s patches and lamina propria were prepared for cytofluorometric analysis of plasma cells and T lymphocytes. The CD4+ T cells were labeled for the intracellular IgA-producing interleukins (ILs)-4, -5, -6, and -10; transforming growth factor (TGF)-β; and the inflammatory cytokines tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and IL-12. In the intestinal tissue samples, myeloperoxidase (MPO) visualization and the enzymatic activity were assessed by immunohistochemistry and ELISA, respectively. The data were analyzed by Student’s t test, and the differences were considered significant at p #x3c; 0.05. Results: In the vagotomy group, the IgA levels and the CD4+ T cells labeled with mediators that promote IgA secretion, including IL-4 (only at lamina propria), TNF-α, and IFN-γ, were decreased, whereas the lamina propria IgA+ plasma cells and MPO presence/activity were increased; changes in the IgM levels, IgM+ plasma cells, and CD4+ T cells labeled with TGF-β, which have a role in class switch recombination, were not observed. Conclusion: The downmodulating impact of vagotomy on IgA levels may result from defective IgA secretion without affecting class switch recombination, whereas vagotomy evoked a proinflammatory response regarding MPO. These findings may reflect the role of the vagus nerve on the control of the IgA response in the small intestine.
Neuroimmunomodulation 2019;26:292–300

中文翻译：

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后dia下迷走神经切断术下调BALB / c小鼠小肠中的IgA水平。

目的：迷走神经后躯干支配整个小肠，阐明其在IgA反应中的调节作用是本研究的目的。方法：两组每只六只雄性BALB / c小鼠在手术后第14天接受假手术或dia下后迷走神经切断术，并处以安乐死。然后，解剖小肠。收集肠液用于通过ELISA的抗体分析，并制备来自Peyer氏斑和固有层的细胞悬液以用于浆细胞和T淋巴细胞的细胞荧光分析。CD4 + T细胞标记有细胞内产生IgA的白介素（ILs）-4，-5，-6和-10；转化生长因子（TGF）-β；炎性细胞因子肿瘤坏死因子（TNF）-α，干扰素（IFN）-γ和IL-12。在肠道组织样本中，分别通过免疫组织化学和ELISA评估了髓过氧化物酶（MPO）的可视化和酶活性。这些数据是由学生的分析ŧ测试，并且在p＃x3c处差异被认为是显着的；0.05。结果：在迷走神经切断术组中，IgA水平和用促进IgA分泌的介质标记的CD4 + T细胞，包括IL-4（仅在固有层），TNF-α和IFN-γ降低，而固有层IgA +浆细胞和MPO的存在/活性增加；没有观察到在类开关重组中起作用的IgM水平，IgM +浆细胞和用TGF-β标记的CD4 + T细胞的变化。结论：迷走神经切断术对IgA水平的下调影响可能是由于IgA分泌缺陷而没有影响类别转换重组，而迷走神经切断术引起了有关MPO的促炎反应。这些发现可能反映了迷走神经在小肠IgA反应控制中的作用。
神经免疫调节2019; 26：292–300