The isolation of a single yeast strain in the clade containing Metschnikowia dekortorum, in the Amazon biome of Brazil, incited us to re-examine the species boundaries within the clade. The strain (UFMG-CM-Y6306) was difficult to position relative to neighbouring species using standard barcode sequences (ITS-D1/D2 rRNA gene region). Mating took place freely with α strains of M. bowlesiae, M. dekortorum, and M. similis, but two-spored asci, indicative of a fertile meiotic progeny, were formed abundantly only with certain strains of M. dekortorum. Accordingly, we examined mating success among every phylotype in the clade and constructed a phylogeny based on a concatenation of 100 of the largest orthologous genes annotated in draft genomes. The analyses confirmed membership of the Amazonian isolate in M. dekortorum, but also indicated that the species should be subdivided into two. As a result, we retain three original members of M. dekortorum in the species, together with the new isolate, and reassign six isolates recovered from Mesoamerican lacustrine habitats to Metschnikowia lacustris sp. nov. The type is UWOPS 12-619.2T (isotype CBS 16250T). MycoBank: MB 833751.

中文翻译：

---

在行为2中捕捉物种：Metschnikowia lacustris sp.。11月，是Metschnikowia dekortorum的姐妹种。

在巴西的亚马逊生物群落中，分离出一个含有Metschnikowia dekortorum的进化枝中的单一酵母菌株，这促使我们重新审视进化枝中的物种边界。使用标准条形码序列（ITS-D1 / D2 rRNA基因区域），菌株（UFMG-CM-Y6306）很难相对于邻近物种定位。交配可与鲍氏支原体，德氏支原体和西里氏支原体的α菌株自由交配，但只有某些菌株的德氏支原体才能大量形成两孢子的asci，表明可育的减数分裂后代。因此，我们检查了进化枝中每个系统型之间的交配成功，并基于在草图基因组中注释的100个最大直系同源基因的串联构建了系统发育。分析证实了M. dekortorum中的亚马逊分离株的成员身份，但也指出该物种应细分为两个。结果，我们在该物种中保留了3个原始的M. dekortorum成员以及新的分离株，并将从中美洲湖生栖息地中回收的6个分离株重新分配给Metschnikowia lacustris sp。十一月 类型为UWOPS 12-619.2T（同型CBS 16250T）。MycoBank：MB 833751。