Osteogenic differentiation of human adipose tissue-derived stem cells (hASCs) is a complex process that is regulated by multiple factors, including microRNAs (miRNAs). The miRNA miR-20a was shown to promote bone formation from bone marrow-derived mesenchymal stem cells. However, the role of miR-20a in osteogenic differentiation of hASCs remains unclear. In this study, we systematically evaluated the function of miR-20a in regulating hASC osteogenesis in vitro. hASCs were transduced with miR-20a-overexpressing and miR-20a-sponge lentiviral vectors, with green fluorescent protein (GFP) as a control. The results showed that miR-20a transcription was upregulated after hASC mineralization. Compared with the miR-20a-sponge, GFP, and hASC groups, the miR-20a-overexpressing group showed higher alkaline phosphatase (ALP) activity on days 7 and 14. Moreover, the mRNA level of ALP increased significantly in the miR-20a-overexpressing group on day 14. Furthermore, the protein of the target gene PPARγ was decreased, and the osteogenic differentiation-associated proteins ALP, osteocalcin, and RUNX2 were upregulated. hASCs anchored to HA/β-TCP revealed a healthy polygonal morphology and developed cytoplasmic extensions. miR-20a promoted osteogenic differentiation of the cell scaffold. Taken together, these data confirm that miRNA-20a promotes the osteogenesis of hASCs in vitro, and its essential role in vivo needs further investigation.

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MicroRNA-20a对人脂肪组织干细胞成骨分化的影响

人类脂肪组织来源的干细胞 (hASCs) 的成骨分化是一个复杂的过程，受多种因素的调控，包括 microRNAs (miRNAs)。miRNA miR-20a 被证明可以促进骨髓间充质干细胞的骨形成。然而，miR-20a 在 hASCs 成骨分化中的作用仍不清楚。在这项研究中，我们系统地评估了 miR-20a 在体外调节 hASC 成骨中的功能。用 miR-20a 过表达和 miR-20a-海绵慢病毒载体转导 hASC，绿色荧光蛋白 (GFP) 作为对照。结果表明 miR-20a 转录在 hASC 矿化后上调。与 miR-20a-sponge、GFP 和 hASC 组相比，miR-20a 过表达组在第 7 天和第 14 天表现出更高的碱性磷酸酶 (ALP) 活性。此外，miR-20a过表达组在第14天ALP的mRNA水平显着升高。此外，靶基因PPARγ的蛋白降低，成骨分化相关蛋白ALP、骨钙素和RUNX2上调。锚定在 HA/β-TCP 上的 hASC 显示出健康的多边形形态并形成细胞质延伸。miR-20a 促进细胞支架的成骨分化。总之，这些数据证实miRNA-20a在体外促进hASCs的成骨，其在体内的重要作用需要进一步研究。锚定在 HA/β-TCP 上的 hASC 显示出健康的多边形形态并形成细胞质延伸。miR-20a 促进细胞支架的成骨分化。总之，这些数据证实miRNA-20a在体外促进hASCs的成骨，其在体内的重要作用需要进一步研究。锚定在 HA/β-TCP 上的 hASC 显示出健康的多边形形态并形成细胞质延伸。miR-20a 促进细胞支架的成骨分化。总之，这些数据证实miRNA-20a在体外促进hASCs的成骨，其在体内的重要作用需要进一步研究。