Eukaryotic messenger RNA (mRNA) is modified by the addition of an inverted guanosine cap to the 5' triphosphate. The cap guanosine and initial transcribed nucleotides are further methylated by a series of cap methyltransferases to generate the mature cap structures which protect RNA from degradation and recruit proteins involved in RNA processing and translation. Research demonstrating that the cap methyltransferases are regulated has generated interest in determining the methylation status of the mRNA cap structures present in cells. Here, we present CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive method for detecting cap structures present in mRNA isolated from tissues or cultured cells.

中文翻译：

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CAP-MAP：帽分析协议，只需最少的分析物处理，即可快速，灵敏地分析mRNA帽结构。

真核生物信使RNA（mRNA）通过在5'三磷酸中添加反向鸟苷帽来修饰。帽鸟苷和最初转录的核苷酸被一系列帽甲基转移酶进一步甲基化，以生成成熟的帽结构，从而保护RNA免受降解并募集参与RNA加工和翻译的蛋白质。证明帽甲基转移酶受到调控的研究引起了人们对确定细胞中存在的mRNA帽结构的甲基化状态的兴趣。在这里，我们介绍CAP-MAP：具有最少分析物处理能力的瓶盖分析方案，一种快速灵敏的方法，用于检测从组织或培养细胞中分离的mRNA中存在的瓶盖结构。