New substituted 1,4-naphthoquinones have been prepared in good overall yields through the naphthol route. The cytotoxicity of these compounds was tested in vitro on MCF-7 breast tumor cells. The most active compound 14 displayed an IC50 of 15μM.

Objective: To investigate the cytotoxicity of new naphthoquinones derivatives on MCF-7 cells.

Methods: Synthesis of new naphtoquinones derivatives and in vitro evaluation of their cytotoxicity on MCF-7 cells (rezasurin cell-based assay).

Results: Starting from Ethyl 4-hydroxy-6,7-dimethoxy-2-naphthoate, four naphthoquinones were prepared and exhibited substantial cytotoxicity against MCF-7 cells.

Conclusion: Preliminary studies of the structure-activity relationship have shown the influence of the structural parameters and, in particular, the nature of the naphthoquinone side chain.

通过萘酚途径已经以良好的总收率制备了新的取代的1，4-萘醌。在体外对MCF-7乳腺肿瘤细胞测试了这些化合物的细胞毒性。活性最高的化合物14的IC50为15μM。

目的：研究新萘醌衍生物对MCF-7细胞的细胞毒性。

方法：合成新的萘醌衍生物，并体外评估其对MCF-7细胞的细胞毒性（基于可瑞沙林的细胞测定）。

结果：从4-羟基-6,7-二甲氧基-2-萘甲酸乙酯开始，制备了四种萘醌，并显示出对MCF-7细胞的实质性细胞毒性。

结论：对结构-活性关系的初步研究显示了结构参数的影响，尤其是萘醌侧链的性质。