Manila clam, Ruditapes philippinarum, is an economically important marine bivalve species. Y-box proteins are members of the cold shock proteins family and highly conserved from bacteria to humans. In this study, a novel Y-box gene (Rpybx) was cloned from the Manila clam and gene expression profiling was performed on three shell color strains (white, zebra and white zebra) and two wild populations (Southern and Northern) of R. philippinarum. The complete ORF length of Rpybx is 1367 bp, encoding 253 amino acids residues. Based on the amino acid sequence analysis and phylogenetic analysis, the Rpybx gene was identified as a member of the invertebrate Y-box proteins family. Rpybx has a similar tertiary structure to human Y-box protein YB-1. The Rpybx mRNA levels were analyzed by qPCR under acute and gradually varied cold stress. Under acute low-temperature stress, the expression of Rpybx mRNA in gills and hepatopancreas was significantly increased in all selected strains and populations (P < 0.05). The Northern population showed the lowest relative expression level of Rpybx. The expressions of Rpybx were greatly upregulated in gills and hepatopancreas of different stains and populations at 5 or -2°C under gradually varied temperature stress (P < 0.05). The results shed light on the biological function of the Rpybx gene in defending against low-temperature challenge and further exploring the molecular mechanism of cold tolerance and resistance in R. philippinarum.

中文翻译：

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低温胁迫下马尼拉蛤Y-box基因（Rpybx）的分子克隆，鉴定及其在不同菌株中的表达分析。

马尼拉蛤，菲律宾蛤仔，是一种经济上重要的海洋双壳类。Y-box蛋白是冷休克蛋白家族的成员，并且从细菌到人类都是高度保守的。在这项研究中，从马尼拉蛤中克隆了一个新的Y-box基因（Rpybx），并对三种壳色菌株（白色，斑马和白斑马）和两个野生R种群（南部和北部）进行了基因表达谱分析。菲律宾。Rpybx的完整ORF长度为1367 bp，编码253个氨基酸残基。根据氨基酸序列分析和系统发育分析，Rpybx基因被鉴定为无脊椎动物Y-box蛋白家族的成员。Rpybx具有类似于人Y盒蛋白YB-1的三级结构。通过qPCR在急性和逐渐变化的冷胁迫下分析Rpybx mRNA水平。在急性低温胁迫下，所有选定菌株和种群中g和肝胰腺中Rpybx mRNA的表达均显着增加（P <0.05）。北部人群显示Rpybx的最低相对表达水平。在逐渐变化的温度胁迫下，在5℃或-2℃，不同染色和种群的g和肝胰腺中Rpybx的表达上调（P <0.05）。该结果揭示了Rpybx基因在防御低温挑战中的生物学功能，并进一步探索了菲律宾墨鱼的耐冷性和抗性的分子机制。北部人群显示Rpybx的最低相对表达水平。在逐渐变化的温度胁迫下，在5℃或-2℃，不同染色和种群的g和肝胰腺中Rpybx的表达上调（P <0.05）。该结果揭示了Rpybx基因在防御低温挑战中的生物学功能，并进一步探索了菲律宾墨鱼的耐冷性和抗性的分子机制。北部人群显示Rpybx的最低相对表达水平。在逐渐变化的温度胁迫下，在5℃或-2℃，不同染色和种群的g和肝胰腺中Rpybx的表达上调（P <0.05）。该结果揭示了Rpybx基因在防御低温挑战中的生物学功能，并进一步探索了菲律宾墨鱼的耐冷性和抗性的分子机制。