In PubMed, it is possible to find more than 40,000 papers on embryo evaluation in various species. However, there is no consensus or gold standard method on how to assess their developmental potential. In assisted reproduction the evaluation "problem" is not only limited to embryos but involves the gametes as well. This manuscript provides an overview of some possible applications of label-free microscopy, in particular we describe the potential of the holographic microscopy in the IVF lab. We describe the positive aspects of several currently available microscopy label-free systems. In conclusion, we believe that a next generation of microscopy able to give objective markers for gamete and embryo quality is around the corner.

中文翻译：

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无标记显微镜：一种评估配子和胚胎质量的非侵入性新工具

在 PubMed 中，可以找到 40,000 多篇关于各种物种胚胎评估的论文。然而，关于如何评估他们的发展潜力并没有达成共识或黄金标准方法。在辅助生殖中，评估“问题”不仅限于胚胎，还涉及配子。这份手稿概述了无标签显微镜的一些可能应用，特别是我们描述了 IVF 实验室中全息显微镜的潜力。我们描述了几种当前可用的显微镜​​无标记系统的积极方面。总之，我们相信能够为配子和胚胎质量提供客观标记的下一代显微镜即将到来。