Polystyrene-block-polyethylene oxide (PS-b-PEO) coated surfaces have been explored as cell culture substrates in the past decade. However, their cytocompatibility has not been extensively assessed. In this study, the in vitro cytocompatibility of PS-b-PEO was investigated. Cellular morphology, metabolic activity, and viability were evaluated at 1, 3, and 5 days after cell seeding. Viability was greater than 90% throughout the 5 days culture, with abundant cell spreading evident by the formation of prominent F-actin stress fibres. The cytocompatibility study was complemented by the analysis of adsorption of a range of extracellular matrix proteins on PS-b-PEO thin films by quartz crystal microbalance with dissipation. Protein adsorption tests revealed that there was no significant difference in protein adhesion between surfaces with a PEO domain coverage of ≈28%, compared to the homogeneous polystyrene control. The findings demonstrate that PS-b-PEO thin films are cytocompatible and are a favourable surface coating for cell culture studies.

中文翻译：

---

聚苯乙烯嵌段聚环氧乙烷薄膜：体外细胞相容性和蛋白质吸附测试。

在过去的十年中，已经探索了聚苯乙烯嵌段聚环氧乙烷（PS-b-PEO）涂层表面作为细胞培养基质。但是，它们的细胞相容性尚未得到广泛评估。在这项研究中，PS-b-PEO的体外细胞相容性进行了研究。在细胞接种后第1、3和5天评估细胞形态，代谢活性和生存力。在整个5天的培养过程中，生存力均大于90％，通过形成明显的F-肌动蛋白应激纤维，细胞扩散明显。细胞相容性研究通过石英晶体微量天平与耗散对PS-b-PEO薄膜上一系列细胞外基质蛋白的吸附分析而得到补充。蛋白质吸附测试表明，与均质聚苯乙烯对照相比，PEO域覆盖率约为28％的表面之间的蛋白质粘附力没有显着差异。这些发现表明PS-b-PEO薄膜具有细胞相容性，是细胞培养研究的有利表面涂层。