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Comparison of the adhesion and endocytosis of calcium oxalate dihydrate to HK-2 cells before and after repair by Astragalus polysaccharide
Science and Technology of Advanced Materials ( IF 5.5 ) Pub Date : 2019-12-31 , DOI: 10.1080/14686996.2019.1697857
Jin Han 1 , Da Guo 2 , Xin-Yuan Sun 2 , Jian-Min Wang 2 , Jian-Ming Ouyang 2 , Bao-Song Gui 1
Affiliation  

ABSTRACT This work investigated the effects of repairing injured renal proximal tubular epithelial (HK-2) cells by using three Astragalus polysaccharides (APS) with different molecular weights and the adhesion and endocytosis of HK-2 cells to the calcium oxalate dihydrate (COD) nanocrystals before and after repair to develop new products that can protect against kidney stones. HK-2 cells cultured in vitro were injured with 2.6 mmol/L oxalic acid to establish a damaged cell model. Three kinds of APS (APS0, APS1, and APS2 with molecular weights of 11.03, 4.72, and 2.60 kDa, respectively) were used to repair the damaged cells. The changes in the adhesion and endocytosis of 100 nm COD crystals to cells before and after the repair were detected. After the repair of HK-2 cells by the APS, the speed of wound healing of the damaged HK-2 cells increased, and the amount of phosphatidylserine (PS) ectropion decreased. In addition, the proportion of cells with adhered COD crystals decreased, whereas the proportion of cells with internalized crystals increased. As a result of the repair activity, APS can inhibit the adhesion and promote the endocytosis of COD nanocrystals to damaged cells. APS1, which had a moderate molecular weight, displayed the strongest abilities to repair the cells, inhibit adhesion, and promote endocytosis. Thus, APS, particularly APS1, may serve as potential green drugs for preventing kidney stones. GRAPHICAL ABSTRACT

中文翻译:

黄芪多糖修复前后二水草酸钙对HK-2细胞黏附和内吞作用的比较

摘要 这项工作研究了使用三种不同分子量的黄芪多糖 (APS) 修复受损肾近端小管上皮 (HK-2) 细胞的效果以及 HK-2 细胞对二水草酸钙 (COD) 纳米晶体的粘附和内吞作用。修复前后开发可预防肾结石的新产品。体外培养的HK-2细胞用2.6 mmol/L草酸损伤,建立损伤细胞模型。三种APS(分子量分别为11.03、4.72和2.60 kDa的APS0、APS1和APS2)用于修复受损细胞。检测修复前后100 nm COD晶体对细胞的粘附和内吞作用的变化。APS修复HK-2细胞后,受损HK-2细胞伤口愈合速度加快,和磷脂酰丝氨酸(PS)的外翻量减少。此外,粘附COD晶体的细胞比例减少,而具有内化晶体的细胞比例增加。由于具有修复活性,APS可以抑制COD纳米晶对受损细胞的粘附并促进其内吞作用。APS1 具有中等分子量,显示出最强的修复细胞、抑制粘附和促进内吞作用的能力。因此,APS,尤其是APS1,可以作为预防肾结石的潜在绿色药物。图形概要 APS可以抑制COD纳米晶对受损细胞的粘附并促进其内吞作用。APS1 具有中等分子量,显示出最强的修复细胞、抑制粘附和促进内吞作用的能力。因此,APS,尤其是APS1,可以作为预防肾结石的潜在绿色药物。图形概要 APS可以抑制COD纳米晶对受损细胞的粘附并促进其内吞作用。APS1 具有中等分子量,显示出最强的修复细胞、抑制粘附和促进内吞作用的能力。因此,APS,尤其是APS1,可以作为预防肾结石的潜在绿色药物。图形概要
更新日期:2019-12-31
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