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Advancing ecological risk assessment on genetically engineered breeding stacks with combined insect-resistance traits.
Transgenic Research ( IF 3 ) Pub Date : 2020-01-17 , DOI: 10.1007/s11248-019-00185-8
Justin McDonald 1 , Andrea Burns 1 , Alan Raybould 2
Affiliation  

To inform the ecological risk assessment (ERA) of a transgenic crop with multiple insecticidal traits combined by conventional breeding (breeding stack), a comparative field study is customarily conducted to compare transgenic protein concentrations in a breeding stack to those in corresponding component single events used in the breeding process. This study tests the hypothesis that transgenic protein expression will not significantly increase due to stacking, such that existing margins of exposure erode to unacceptable levels. Corroboration of this hypothesis allows for the use of existing non-target organism (NTO) effects tests results, where doses were based on the estimated environmental concentrations determined for a component single event. Results from over 20 studies comparing expression profiles of insecticidal proteins produced by commercial events in various combinations of conventionally-bred stacks were examined to evaluate applying previously determined no-observed-effect concentrations (NOECs) to stack ERAs. This paper presents a large number of tests corroborating the hypothesis of no significant increase in insecticidal protein expression due to combination by conventional breeding, and much of the variation in protein expression is likely attributed to genetic and environmental factors. All transgenic protein concentrations were well within conservative margins between exposure and corresponding NOEC. This work supports the conclusion that protein expression data generated for single events and the conservative manner for setting NTO effects test concentrations allows for the transportability of existing NOECs to the ERA of conventionally-bred stacks, and that future tests of the stated hypothesis are no longer critically informative for ERA on breeding stacks.

中文翻译:

推进具有综合抗虫性状的基因工程育种栈的生态风险评估。

为了向具有常规育种(育种堆栈)的多种杀虫性状的转基因作物提供生态风险评估(ERA),通常进行比较田间研究,以比较育种堆栈中的转基因蛋白质浓度与所使用的相应成分单一事件中的转基因蛋白质浓度。在繁殖过程中。这项研究检验了以下假设:转基因蛋白表达不会因堆积而显着增加,从而使现有的暴露裕度降低到不可接受的水平。该假设的确证允许使用现有的非目标生物(NTO)效果测试结果,其中剂量基于为单个事件确定的估计环境浓度。检查了20多个研究结果,比较了由商业事件在常规饲养烟囱的各种组合中通过商业活动产生的杀虫蛋白的表达谱,以评估将先前确定的无观察到的浓度(NOEC)应用于烟囱ERA的情况。本文提出了大量试验,以证实以下假设:由于常规育种的结合,杀虫蛋白表达没有显着增加,并且蛋白表达的许多变化很可能归因于遗传和环境因素。所有转基因蛋白浓度均在暴露与相应NOEC之间的保守范围内。
更新日期:2020-01-17
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