D-Allulose, a C-3 epimer of D-fructose, is a rare sugar and a non-caloric sweetener. D-Allulose is reported to have several health benefits, such as suppressing a rise in postprandial glucose levels and preventing fat accumulation in rodents and humans. Additionally, low HDL-cholesterol levels post-D-allulose feeding were observed in humans but it is unclear how D-allulose decreased HDL-cholesterol levels. It is necessary to research the mechanism of HDL-cholesterol reduction by D-allulose ingestion because low HDL-cholesterol levels are known to associate with increased atherosclerosis risk. We therefore investigated the mechanism by which D-allulose lowers HDL-cholesterol using rat's primary hepatocytes. Sprague Dawley rats were fed an AIN-93G based diet containing 3% D-allulose for 2 weeks. Thereafter, primary hepatocytes were isolated by perfusion of collagenase. We measured the ability of HDL-cholesterol uptake in hepatocytes and the protein levels of scavenger receptor class B type 1 (SR-B1) as a HDL-cholesterol receptor. D-Allulose enhanced hepatocyte uptake of HDL-cholesterol, with a concurrent increase in hepatic SR-B1 protein levels. The results suggest that D-allulose enhances HDL-cholesterol uptake into the liver by increasing SR-B1 expression. It is estimated that HDL-cholesterol levels decreased accordingly. Since SR-B1 overexpression would decrease HDL-cholesterol levels, reportedly preventing atherosclerosis development, D-allulose could be a useful sweetener for atherosclerosis prevention.

中文翻译：

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D-Allulose可通过SR-B1增强HDL-胆固醇对大鼠原代肝细胞的吸收。

D-果糖，D-果糖的C-3差向异构体，是一种稀有的糖，也是一种无热量的甜味剂。据报道，D-阿洛糖具有多种健康益处，例如抑制餐后葡萄糖水平的升高以及防止啮齿动物和人类中脂肪的积累。另外，在人类中观察到D-阿洛糖喂养后HDL-胆固醇水平低，但是尚不清楚D-阿洛糖如何降低HDL-胆固醇水平。有必要研究D-阿洛糖摄入减少HDL-胆固醇的机制，因为已知低水平的HDL-胆固醇会增加动脉粥样硬化的风险。因此，我们使用大鼠原代肝细胞研究了D-阿洛糖降低HDL-胆固醇的机制。将含有3％D-阿洛糖的AIN-93G饮食喂养Sprague Dawley大鼠2周。之后，通过灌注胶原酶分离原代肝细胞。我们测量了肝细胞摄取HDL-胆固醇的能力以及作为HDL-胆固醇受体的B类1类清道夫受体的蛋白水平。D-Allulose增强了HDL-胆固醇对肝细胞的吸收，并同时增加了肝SR-B1蛋白水平。结果表明，D-阿洛糖通过增加SR-B1表达来增强HDL-胆固醇对肝脏的吸收。据估计，HDL-胆固醇水平相应降低。由于SR-B1过表达会降低HDL-胆固醇水平，据报道可预防动脉粥样硬化的发生，因此D-阿洛糖可能是预防动脉粥样硬化的有用甜味剂。我们测量了肝细胞摄取HDL-胆固醇的能力以及作为HDL-胆固醇受体的B类1类清道夫受体的蛋白水平。D-Allulose增强了HDL-胆固醇对肝细胞的吸收，并同时增加了肝SR-B1蛋白水平。结果表明，D-阿洛糖通过增加SR-B1表达来增强HDL-胆固醇对肝脏的吸收。据估计，HDL-胆固醇水平相应降低。由于SR-B1过表达会降低HDL-胆固醇水平，据报道可预防动脉粥样硬化的发生，因此D-阿洛糖可能是预防动脉粥样硬化的有用甜味剂。我们测量了肝细胞摄取HDL-胆固醇的能力以及作为HDL-胆固醇受体的B类1类清道夫受体的蛋白水平。D-Allulose增强了HDL-胆固醇对肝细胞的吸收，并同时增加了肝SR-B1蛋白水平。结果表明，D-阿洛糖通过增加SR-B1表达来增强HDL-胆固醇对肝脏的吸收。据估计，HDL-胆固醇水平相应降低。由于SR-B1过表达会降低HDL-胆固醇水平，据报道可预防动脉粥样硬化的发生，因此D-阿洛糖可能是预防动脉粥样硬化的有用甜味剂。结果表明，D-阿洛糖通过增加SR-B1表达来增强HDL-胆固醇对肝脏的吸收。据估计，HDL-胆固醇水平相应降低。由于SR-B1过表达会降低HDL-胆固醇水平，据报道可预防动脉粥样硬化的发生，因此D-阿洛糖可能是预防动脉粥样硬化的有用甜味剂。结果表明，D-阿洛糖通过增加SR-B1表达来增强HDL-胆固醇对肝脏的吸收。据估计，HDL-胆固醇水平相应降低。由于SR-B1过表达会降低HDL-胆固醇水平，据报道可预防动脉粥样硬化的发生，因此D-阿洛糖可能是预防动脉粥样硬化的有用甜味剂。