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Impact of cochlear ablation on calretinin and synaptophysin in the gerbil anteroventral cochlear nucleus before the hearing onset
Journal of Chemical Neuroanatomy ( IF 2.8 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.jchemneu.2020.101746
Ivonne Bazwinsky-Wutschke 1 , Faramarz Dehghani 2
Affiliation  

Mammalian auditory system undergoes many structural and functional modifications during postnatal development, which are dependent on the relationship between auditory nerve fibers and their nuclei. In the present study, the cochlea of Meriones unguiculatus was ablated unilaterally on postnatal day 5 or 9 (P5 or P9), before the onset of hearing. Histochemical analysis of synaptophysin (SYN) and calretinin (CR) in anterior anteroventral cochlear nucleus (AVCN-A) was performed to analyze whether unilateral cochlea ablation induces changes in the auditory terminal endings and somata of spherical bushy cells (SBCs). During the period of postnatal development, CR-labeling was evident in somata of SBCs and in auditory nerve terminals. SYN was most apparent in puncta encircled cell bodies, progressing with age. Cochlear removal at P5 induced a decrease in CR-labeling in SBCs somata 6 h and 48 h post-lesion; whereas, ablation at P9 increased the somatic CR-labeling in the lesioned AVCN-A after 24 and 48 h post-lesion. The SYN-labeled synaptic puncta were remarkably reduced in the AVCN-A of P5- and P9-cochlea-ablated gerbils with stronger effects in P5 animals (a 50% reduction after 48 h). Interestingly, a significant increase in the SYN-immunolabeled puncta was found after 48 h compared to 24 h in the lesioned AVCN-A of P9 gerbils, indicating reactive synaptogenesis. Our study shows, that following the destruction of the cochlea at different postnatal periods, the CR- and SYN-labeling are differentially influenced in the AVCN-A, which in turn coincides with different critical developmental periods before the onset of hearing.

中文翻译:

耳蜗消融对听力开始前沙鼠前腹耳蜗核钙视网膜蛋白和突触素的影响

哺乳动物的听觉系统在出生后发育过程中经历了许多结构和功能的改变,这取决于听觉神经纤维与其细胞核之间的关系。在本研究中,在出生后第 5 天或第 9 天(P5 或 P9),在听力开始之前,单侧耳蜗消融了 Meriones unguiculatus。对前耳蜗前核 (AVCN-A) 中的突触素 (SYN) 和钙视网膜蛋白 (CR) 进行组织化学分析,以分析单侧耳蜗消融是否会导致球形浓密细胞 (SBC) 的听觉末梢和胞体发生变化。在出生后发育期间,SBC 的胞体和听神经末梢中明显存在 CR 标记。SYN在点状环绕的细胞体中最为明显,随着年龄的增长而进展。在 P5 时去除耳蜗会导致损伤后 6 小时和 48 小时 SBC 胞体中 CR 标记的减少;而在 P9 的消融增加了损伤后 24 和 48 小时后损伤 AVCN-A 中的体细胞 CR 标记。SYN 标记的突触点在 P5 和 P9 耳蜗消融沙鼠的 AVCN-A 中显着减少,对 P5 动物的影响更强(48 小时后减少 50%)。有趣的是,与 P9 沙鼠的受损 AVCN-A 中的 24 小时相比,48 小时后发现 SYN 免疫标记的斑点显着增加,表明反应性突触发生。我们的研究表明,随着出生后不同时期耳蜗的破坏,AVCN-A 中的 CR 和 SYN 标记受到不同的影响,这反过来又与听力开始前的不同关键发育时期相吻合。
更新日期:2020-03-01
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